Plasma proteome analysis of patients with type 1 diabetes with diabetic nephropathy
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Plasma proteome analysis of patients with type 1 diabetes with diabetic nephropathy. / Overgaard, Anne Julie; Hansen, Henning Gram; Lajer, Maria; Pedersen, Lykke; Tarnow, Lise; Rossing, Peter; McGuire, James N.; Pociot, Flemming.
I: Proteome Science, Bind 8, 2010, s. 4.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Plasma proteome analysis of patients with type 1 diabetes with diabetic nephropathy
AU - Overgaard, Anne Julie
AU - Hansen, Henning Gram
AU - Lajer, Maria
AU - Pedersen, Lykke
AU - Tarnow, Lise
AU - Rossing, Peter
AU - McGuire, James N.
AU - Pociot, Flemming
PY - 2010
Y1 - 2010
N2 - As part of a clinical proteomics program focused on diabetes and its complications we are looking for new and better protein biomarkers for diabetic nephropathy. The search for new and better biomarkers for diabetic nephropathy has, with a few exceptions, previously focused on either hypothesis-driven studies or urinary based investigations. To date only two studies have investigated the proteome of blood in search for new biomarkers, and these studies were conducted in sera from patients with type 2 diabetes. This is the first reported in depth proteomic study where plasma from type 1 diabetic patients was investigated with the goal of finding improved candidate biomarkers to predict diabetic nephropathy. In order to reach lower concentration proteins in plasma a pre-fractionation step, either hexapeptide bead-based libraries or anion exchange chromatography, was performed prior to surface enhanced laser desorption/ionization time-of-flight mass spectrometry analysis.
AB - As part of a clinical proteomics program focused on diabetes and its complications we are looking for new and better protein biomarkers for diabetic nephropathy. The search for new and better biomarkers for diabetic nephropathy has, with a few exceptions, previously focused on either hypothesis-driven studies or urinary based investigations. To date only two studies have investigated the proteome of blood in search for new biomarkers, and these studies were conducted in sera from patients with type 2 diabetes. This is the first reported in depth proteomic study where plasma from type 1 diabetic patients was investigated with the goal of finding improved candidate biomarkers to predict diabetic nephropathy. In order to reach lower concentration proteins in plasma a pre-fractionation step, either hexapeptide bead-based libraries or anion exchange chromatography, was performed prior to surface enhanced laser desorption/ionization time-of-flight mass spectrometry analysis.
U2 - 10.1186/1477-5956-8-4
DO - 10.1186/1477-5956-8-4
M3 - Journal article
C2 - 20205888
VL - 8
SP - 4
JO - Proteome Science
JF - Proteome Science
SN - 1477-5956
ER -
ID: 32671884