Photochemical crosslinking of protein and DNA in chromatin. II. Synthesis and application of psoralen-cystamine-arylazido photocrosslinking reagents
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
The synthesis and testing of a new type of nucleic acid-protein photocrosslinking reagent is described. The reagents are composed of a psoralen ligand for nucleic acid photoattachment, which is linked to an azidobenzoyl group, for protein photoattachment. The linker contains a disulfide bridge which can be opened by reduction with mercaptans. The reagents were tested in a chromatin system, where it was found that they induced cleavable crosslinks between the histones and the DNA upon irradiation with long-wavelength ultraviolet light (lambda greater than 300 nm).
Originalsprog | Engelsk |
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Tidsskrift | Analytical Biochemistry |
Vol/bind | 149 |
Udgave nummer | 2 |
Sider (fra-til) | 575-81 |
Antal sider | 7 |
ISSN | 0003-2697 |
DOI | |
Status | Udgivet - sep. 1985 |
ID: 203631738