Persistence and genetic adaptation of Pseudomonas aeruginosa in patients with chronic obstructive pulmonary disease

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Persistence and genetic adaptation of Pseudomonas aeruginosa in patients with chronic obstructive pulmonary disease. / Eklöf, Josefin; Misiakou, Maria A.; Sivapalan, Pradeesh; Armbruster, Karin; Browatzki, Andrea; Nielsen, Thyge L.; Lapperre, Therese S.; Andreassen, Helle F.; Janner, Julie; Ulrik, Charlotte S.; Gabrielaite, Migle; Johansen, Helle K.; Jensen, Annemette; Nielsen, Tine V.; Hertz, Frederik B.; Ghathian, Khaled; Calum, Henrik; Wilcke, Torgny; Seersholm, Niels; Jensen, Jens Ulrik S.; Marvig, Rasmus L.

I: Clinical Microbiology and Infection, Bind 28, Nr. 7, 2022, s. 990-995.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Eklöf, J, Misiakou, MA, Sivapalan, P, Armbruster, K, Browatzki, A, Nielsen, TL, Lapperre, TS, Andreassen, HF, Janner, J, Ulrik, CS, Gabrielaite, M, Johansen, HK, Jensen, A, Nielsen, TV, Hertz, FB, Ghathian, K, Calum, H, Wilcke, T, Seersholm, N, Jensen, JUS & Marvig, RL 2022, 'Persistence and genetic adaptation of Pseudomonas aeruginosa in patients with chronic obstructive pulmonary disease', Clinical Microbiology and Infection, bind 28, nr. 7, s. 990-995. https://doi.org/10.1016/j.cmi.2022.01.017

APA

Eklöf, J., Misiakou, M. A., Sivapalan, P., Armbruster, K., Browatzki, A., Nielsen, T. L., Lapperre, T. S., Andreassen, H. F., Janner, J., Ulrik, C. S., Gabrielaite, M., Johansen, H. K., Jensen, A., Nielsen, T. V., Hertz, F. B., Ghathian, K., Calum, H., Wilcke, T., Seersholm, N., ... Marvig, R. L. (2022). Persistence and genetic adaptation of Pseudomonas aeruginosa in patients with chronic obstructive pulmonary disease. Clinical Microbiology and Infection, 28(7), 990-995. https://doi.org/10.1016/j.cmi.2022.01.017

Vancouver

Eklöf J, Misiakou MA, Sivapalan P, Armbruster K, Browatzki A, Nielsen TL o.a. Persistence and genetic adaptation of Pseudomonas aeruginosa in patients with chronic obstructive pulmonary disease. Clinical Microbiology and Infection. 2022;28(7):990-995. https://doi.org/10.1016/j.cmi.2022.01.017

Author

Eklöf, Josefin ; Misiakou, Maria A. ; Sivapalan, Pradeesh ; Armbruster, Karin ; Browatzki, Andrea ; Nielsen, Thyge L. ; Lapperre, Therese S. ; Andreassen, Helle F. ; Janner, Julie ; Ulrik, Charlotte S. ; Gabrielaite, Migle ; Johansen, Helle K. ; Jensen, Annemette ; Nielsen, Tine V. ; Hertz, Frederik B. ; Ghathian, Khaled ; Calum, Henrik ; Wilcke, Torgny ; Seersholm, Niels ; Jensen, Jens Ulrik S. ; Marvig, Rasmus L. / Persistence and genetic adaptation of Pseudomonas aeruginosa in patients with chronic obstructive pulmonary disease. I: Clinical Microbiology and Infection. 2022 ; Bind 28, Nr. 7. s. 990-995.

Bibtex

@article{fcaccb6ff0e347738470bf36abbd5aa1,

title = "Persistence and genetic adaptation of Pseudomonas aeruginosa in patients with chronic obstructive pulmonary disease",

abstract = "Objectives: It is unclear whether recurrent sputum culture with Pseudomonas aeruginosa from patients with chronic obstructive pulmonary disease (COPD) is caused by intermittent airway carriage by different P. aeruginosa lineages or persistent carriage by the same lineage, and whether lineages genetically adapt during carriage. Methods: Whole-genome sequencing was performed for P. aeruginosa isolates sampled longitudinally from sputum cultures in patients with COPD who were enrolled in an ongoing randomized controlled trial (clinicaltrials.gov: NCT03262142). Results: A total of 153 P. aeruginosa isolates were sequenced for 23 patients during 365 days of follow-up. Recurrent presence of P. aeruginosa was seen in 19 patients (83%) and was caused by persistence of the same clonal lineage in all but one patient. We identified 38 genes mutated in parallel in two or more lineages, suggesting positive selection for adaptive mutations. Mutational enrichment analysis revealed genes important in antibiotic resistance and chronic infections to be more frequently mutated. Discussion: Recurrent P. aeruginosa was common and carried for a prolonged time after initial detection in the airways of patients with COPD. Recurrence was caused by persistence of the same clonal lineage and was associated with genetic adaptation. Trial data on possible clinical benefits of attempting antibiotic eradication of P. aeruginosa in COPD are warranted.",

keywords = "Chronic obstructive pulmonary disease, Genetic adaptation, Pseudomonas aeruginosa, Whole-genome sequencing",

author = "Josefin Ekl{\"o}f and Misiakou, {Maria A.} and Pradeesh Sivapalan and Karin Armbruster and Andrea Browatzki and Nielsen, {Thyge L.} and Lapperre, {Therese S.} and Andreassen, {Helle F.} and Julie Janner and Ulrik, {Charlotte S.} and Migle Gabrielaite and Johansen, {Helle K.} and Annemette Jensen and Nielsen, {Tine V.} and Hertz, {Frederik B.} and Khaled Ghathian and Henrik Calum and Torgny Wilcke and Niels Seersholm and Jensen, {Jens Ulrik S.} and Marvig, {Rasmus L.}",

note = "Publisher Copyright: {\textcopyright} 2022 The Author(s)",

year = "2022",

doi = "10.1016/j.cmi.2022.01.017",

language = "English",

volume = "28",

pages = "990--995",

journal = "Clinical Microbiology and Infection",

issn = "1198-743X",

publisher = "Elsevier",

number = "7",

}

RIS

TY - JOUR

T1 - Persistence and genetic adaptation of Pseudomonas aeruginosa in patients with chronic obstructive pulmonary disease

AU - Eklöf, Josefin

AU - Misiakou, Maria A.

AU - Sivapalan, Pradeesh

AU - Armbruster, Karin

AU - Browatzki, Andrea

AU - Nielsen, Thyge L.

AU - Lapperre, Therese S.

AU - Andreassen, Helle F.

AU - Janner, Julie

AU - Ulrik, Charlotte S.

AU - Gabrielaite, Migle

AU - Johansen, Helle K.

AU - Jensen, Annemette

AU - Nielsen, Tine V.

AU - Hertz, Frederik B.

AU - Ghathian, Khaled

AU - Calum, Henrik

AU - Wilcke, Torgny

AU - Seersholm, Niels

AU - Jensen, Jens Ulrik S.

AU - Marvig, Rasmus L.

PY - 2022

Y1 - 2022

N2 - Objectives: It is unclear whether recurrent sputum culture with Pseudomonas aeruginosa from patients with chronic obstructive pulmonary disease (COPD) is caused by intermittent airway carriage by different P. aeruginosa lineages or persistent carriage by the same lineage, and whether lineages genetically adapt during carriage. Methods: Whole-genome sequencing was performed for P. aeruginosa isolates sampled longitudinally from sputum cultures in patients with COPD who were enrolled in an ongoing randomized controlled trial (clinicaltrials.gov: NCT03262142). Results: A total of 153 P. aeruginosa isolates were sequenced for 23 patients during 365 days of follow-up. Recurrent presence of P. aeruginosa was seen in 19 patients (83%) and was caused by persistence of the same clonal lineage in all but one patient. We identified 38 genes mutated in parallel in two or more lineages, suggesting positive selection for adaptive mutations. Mutational enrichment analysis revealed genes important in antibiotic resistance and chronic infections to be more frequently mutated. Discussion: Recurrent P. aeruginosa was common and carried for a prolonged time after initial detection in the airways of patients with COPD. Recurrence was caused by persistence of the same clonal lineage and was associated with genetic adaptation. Trial data on possible clinical benefits of attempting antibiotic eradication of P. aeruginosa in COPD are warranted.

AB - Objectives: It is unclear whether recurrent sputum culture with Pseudomonas aeruginosa from patients with chronic obstructive pulmonary disease (COPD) is caused by intermittent airway carriage by different P. aeruginosa lineages or persistent carriage by the same lineage, and whether lineages genetically adapt during carriage. Methods: Whole-genome sequencing was performed for P. aeruginosa isolates sampled longitudinally from sputum cultures in patients with COPD who were enrolled in an ongoing randomized controlled trial (clinicaltrials.gov: NCT03262142). Results: A total of 153 P. aeruginosa isolates were sequenced for 23 patients during 365 days of follow-up. Recurrent presence of P. aeruginosa was seen in 19 patients (83%) and was caused by persistence of the same clonal lineage in all but one patient. We identified 38 genes mutated in parallel in two or more lineages, suggesting positive selection for adaptive mutations. Mutational enrichment analysis revealed genes important in antibiotic resistance and chronic infections to be more frequently mutated. Discussion: Recurrent P. aeruginosa was common and carried for a prolonged time after initial detection in the airways of patients with COPD. Recurrence was caused by persistence of the same clonal lineage and was associated with genetic adaptation. Trial data on possible clinical benefits of attempting antibiotic eradication of P. aeruginosa in COPD are warranted.

KW - Chronic obstructive pulmonary disease

KW - Genetic adaptation

KW - Pseudomonas aeruginosa

KW - Whole-genome sequencing

U2 - 10.1016/j.cmi.2022.01.017

DO - 10.1016/j.cmi.2022.01.017

M3 - Journal article

C2 - 35124256

AN - SCOPUS:85125441764

VL - 28

SP - 990

EP - 995

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

SN - 1198-743X

IS - 7

ER -

ID: 314075555