Peanut-shaped gold nanoparticles with shells of ceragenin csa-131 display the ability to inhibit ovarian cancer growth in vitro and in a tumor xenograft model

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  • Ewelina Piktel
  • Ilona Oscilowska
  • Łukasz Suprewicz
  • Joanna Depciuch
  • Natalia Marcińczyk
  • Ewa Chabielska
  • Przemysław Wolak
  • Katarzyna Głuszek
  • Justyna Klimek
  • Piotr M. Zieliński
  • Marzec, Michal Tomasz
  • Paul B. Savage
  • Magdalena Parlińska-Wojtan
  • Robert Bucki

Gold nanoparticles-assisted delivery of antineoplastics into cancerous cells is presented as an effective approach for overcoming the limitations of systemic chemotherapy. Although ceragenins show great potential as anti-cancer agents, in some tumors, effective inhibition of cancer cells proliferation requires application of ceragenins at doses within their hemolytic range. For the purpose of toxicity/efficiency ratio control, peanut-shaped gold nanoparticles (AuP NPs) were functionalized with a shell of ceragenin CSA-131 and the cytotoxicity of AuP@CSA-131 against ovarian cancer SKOV-3 cells and were then analyzed. In vivo efficiency of intravenously and intratumorally administered CSA-131 and AuP@CSA-131 was examined using a xenograft ovarian cancer model. Serum parameters were estimated using ELISA methods. Comparative analysis revealed that AuP@CSA-131 exerted stronger anti-cancer effects than free ceragenin, which was determined by enhanced ability to induce caspase-dependent apoptosis and autophagy processes via reactive oxygen species (ROS)-mediated pathways. In an animal study, AuP@CSA-131 was characterized by delayed clearance and prolonged blood circulation when compared with free ceragenin, as well as enhanced anti-tumor efficiency, particularly when applied intratumorally. Administration of CSA-131 and AuP@CSA-131 prevented the inflammatory response associated with cancer development. These results present the possibility of employing non-spherical gold nanoparticles as an effective nanoplatform for the delivery of antineoplastics for the treatment of ovarian malignancy.

Udgave nummer21
Sider (fra-til)1-26
StatusUdgivet - 2021

ID: 284639988