This study presents pathological conditions observed in the clavicular joints of the extinct giant ground sloth Megatherium americanum. Clavicles are key for shoulders and forelimbs movements in both feeding and locomotion, two aspects largely discussed in this species. Consequently, paleopathologies of this bone are a direct proxy for understanding paleoecological issues and provide indirect evidence of the paleoenvironment of this species. Eleven clavicles of Megatherium americanum from the Pleistocene of the Pampean region (Argentina), housed in European museums, have been studied. We analyzed the external and inner structure of the bone through macroscopic observation and imaging techniques (e. g., Computed tomography scanner [CT] and 3D surface scanner) and uncovered subchondral sclerosis, exostosis, cysts, and draining sinuses in seven of the analyzed samples. Our results indicate that the clavicles showed signs of arthritis and infections and were subjected to trauma. Adduction/abduction and superior movements when standing next to trees for feeding, and compressive forces due to quadrupedal locomotion by this species, might have affected the clavicle joints. This suggests that the animals tried to reach the higher levels of arbors, and indirectly implies the presence of edible groves into the grassland Pampean landscape. Based on the paleopathologies of the clavicle, the paleoecological aspects are in line with other ecological studies performed on this species.