Onset of small intestinal atrophy is associated with reduced intestinal blood flow in TPN-fed neonatal piglets
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Onset of small intestinal atrophy is associated with reduced intestinal blood flow in TPN-fed neonatal piglets. / Niinikoski, Harri; Stoll, Barbara; Guan, Xinfu; Kansagra, Ketan; Lambert, Barry D; Stephens, John; Hartmann, Bolette; Holst, Jens Juul; Burrin, Douglas G.
I: Journal of Nutrition, Bind 134, Nr. 6, 06.2004, s. 1467-74.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Onset of small intestinal atrophy is associated with reduced intestinal blood flow in TPN-fed neonatal piglets
AU - Niinikoski, Harri
AU - Stoll, Barbara
AU - Guan, Xinfu
AU - Kansagra, Ketan
AU - Lambert, Barry D
AU - Stephens, John
AU - Hartmann, Bolette
AU - Holst, Jens Juul
AU - Burrin, Douglas G
PY - 2004/6
Y1 - 2004/6
N2 - Our aim was to determine the speed of onset of total parenteral nutrition (TPN)-induced mucosal atrophy, and whether this is associated with changes in intestinal blood flow and tissue metabolism in neonatal piglets. Piglets were implanted with jugular venous and duodenal catheters and either a portal venous or superior mesenteric artery (SMA) blood flow probe. At 3 wk of age, piglets were randomly assigned to receive continuous enteral formula feeding (n = 8) or TPN (n = 17) for 24 or 48 h. Blood flow was recorded continuously and piglets were given an i.v. bolus of bromodeoxyuridine and (13)C-phenylalanine to measure crypt cell proliferation and protein synthesis, respectively. After 8 h of TPN, portal and SMA blood flow decreased 30% compared with enteral feeding (P < 0.01), and remained near levels of food-deprived piglets for the remaining 48 h of TPN. After 24 h, TPN reduced jejunal inducible nitric oxide synthase (iNOS) activity and protein abundance (P < 0.05), small intestinal weight, and villous height (P < 0.01) compared with enterally fed piglets. Cell proliferation and DNA mass were decreased (P < 0.05) and apoptosis increased (P < 0.05) after 48 h of TPN. Protein synthesis was lower (P < 0.05) after 24 h of TPN, and protein mass was lower (P < 0.05) after 48 h of TPN, compared with enteral feeding. These data indicate that the transition from enteral to parenteral nutrition induced a rapid (<8 h) decrease in intestinal blood flow, and this likely precedes villous atrophy and the suppression of protein synthesis at 24 h, and of cell proliferation and survival at 48 h.
AB - Our aim was to determine the speed of onset of total parenteral nutrition (TPN)-induced mucosal atrophy, and whether this is associated with changes in intestinal blood flow and tissue metabolism in neonatal piglets. Piglets were implanted with jugular venous and duodenal catheters and either a portal venous or superior mesenteric artery (SMA) blood flow probe. At 3 wk of age, piglets were randomly assigned to receive continuous enteral formula feeding (n = 8) or TPN (n = 17) for 24 or 48 h. Blood flow was recorded continuously and piglets were given an i.v. bolus of bromodeoxyuridine and (13)C-phenylalanine to measure crypt cell proliferation and protein synthesis, respectively. After 8 h of TPN, portal and SMA blood flow decreased 30% compared with enteral feeding (P < 0.01), and remained near levels of food-deprived piglets for the remaining 48 h of TPN. After 24 h, TPN reduced jejunal inducible nitric oxide synthase (iNOS) activity and protein abundance (P < 0.05), small intestinal weight, and villous height (P < 0.01) compared with enterally fed piglets. Cell proliferation and DNA mass were decreased (P < 0.05) and apoptosis increased (P < 0.05) after 48 h of TPN. Protein synthesis was lower (P < 0.05) after 24 h of TPN, and protein mass was lower (P < 0.05) after 48 h of TPN, compared with enteral feeding. These data indicate that the transition from enteral to parenteral nutrition induced a rapid (<8 h) decrease in intestinal blood flow, and this likely precedes villous atrophy and the suppression of protein synthesis at 24 h, and of cell proliferation and survival at 48 h.
KW - Animals
KW - Animals, Newborn
KW - Apoptosis
KW - Atrophy
KW - Cell Division
KW - DNA
KW - Enteral Nutrition
KW - Female
KW - Food Deprivation
KW - Glucagon-Like Peptides
KW - Intestinal Mucosa
KW - Intestine, Small
KW - Nitric Oxide Synthase
KW - Organ Size
KW - Parenteral Nutrition, Total
KW - Peptides
KW - Portal System
KW - Protein Biosynthesis
KW - Regional Blood Flow
KW - Survival Rate
KW - Swine
KW - Time Factors
M3 - Journal article
C2 - 15173413
VL - 134
SP - 1467
EP - 1474
JO - Journal of Nutrition
JF - Journal of Nutrition
SN - 0022-3166
IS - 6
ER -
ID: 132054370