Non-invasive method for in vivo detection of chlorophyll precursors

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Standard

Non-invasive method for in vivo detection of chlorophyll precursors. / Kristiansen, Kim Anker; Khrouchtchova, Anastassia; Stenbæk, Anne; Schulz, Alexander; Jensen, Poul Erik.

I: Photochemical & Photobiological Sciences, Bind 8, Nr. 2, 2009, s. 279-286.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Kristiansen, KA, Khrouchtchova, A, Stenbæk, A, Schulz, A & Jensen, PE 2009, 'Non-invasive method for in vivo detection of chlorophyll precursors', Photochemical & Photobiological Sciences, bind 8, nr. 2, s. 279-286. https://doi.org/10.1039/b811774h

APA

Kristiansen, K. A., Khrouchtchova, A., Stenbæk, A., Schulz, A., & Jensen, P. E. (2009). Non-invasive method for in vivo detection of chlorophyll precursors. Photochemical & Photobiological Sciences, 8(2), 279-286. https://doi.org/10.1039/b811774h

Vancouver

Kristiansen KA, Khrouchtchova A, Stenbæk A, Schulz A, Jensen PE. Non-invasive method for in vivo detection of chlorophyll precursors. Photochemical & Photobiological Sciences. 2009;8(2):279-286. https://doi.org/10.1039/b811774h

Author

Kristiansen, Kim Anker ; Khrouchtchova, Anastassia ; Stenbæk, Anne ; Schulz, Alexander ; Jensen, Poul Erik. / Non-invasive method for in vivo detection of chlorophyll precursors. I: Photochemical & Photobiological Sciences. 2009 ; Bind 8, Nr. 2. s. 279-286.

Bibtex

@article{14c750600b5011df825d000ea68e967b,
title = "Non-invasive method for in vivo detection of chlorophyll precursors",
abstract = "Traditionally chlorophyll (Chl) and Chl precursors have been studied in vitro or in leaf tissue at low temperature. These methods are destructive and make it impossible to work with the same individual plant later on. In this paper we present a method for in vivo detection of Chl and its precursors in seedling plants which can be rescued for further studies. Multi-photon microscopy, which we show to be more reliable in vivo than UV-laser microscopy, is used to detect precursors in the biosynthetic pathway leading to Chl. The sensitivity and ability to distinguish different precursors with this system is compared to current methods. Furthermore, we report on optimization of the spectral scanning method with the aim to minimize the excitation light-evoked photo-conversion of the chlorophyll precursors.",
author = "Kristiansen, {Kim Anker} and Anastassia Khrouchtchova and Anne Stenb{\ae}k and Alexander Schulz and Jensen, {Poul Erik}",
year = "2009",
doi = "10.1039/b811774h",
language = "English",
volume = "8",
pages = "279--286",
journal = "Photochemical & Photobiological Sciences",
issn = "1474-905X",
publisher = "Royal Society of Chemistry",
number = "2",

}

RIS

TY - JOUR

T1 - Non-invasive method for in vivo detection of chlorophyll precursors

AU - Kristiansen, Kim Anker

AU - Khrouchtchova, Anastassia

AU - Stenbæk, Anne

AU - Schulz, Alexander

AU - Jensen, Poul Erik

PY - 2009

Y1 - 2009

N2 - Traditionally chlorophyll (Chl) and Chl precursors have been studied in vitro or in leaf tissue at low temperature. These methods are destructive and make it impossible to work with the same individual plant later on. In this paper we present a method for in vivo detection of Chl and its precursors in seedling plants which can be rescued for further studies. Multi-photon microscopy, which we show to be more reliable in vivo than UV-laser microscopy, is used to detect precursors in the biosynthetic pathway leading to Chl. The sensitivity and ability to distinguish different precursors with this system is compared to current methods. Furthermore, we report on optimization of the spectral scanning method with the aim to minimize the excitation light-evoked photo-conversion of the chlorophyll precursors.

AB - Traditionally chlorophyll (Chl) and Chl precursors have been studied in vitro or in leaf tissue at low temperature. These methods are destructive and make it impossible to work with the same individual plant later on. In this paper we present a method for in vivo detection of Chl and its precursors in seedling plants which can be rescued for further studies. Multi-photon microscopy, which we show to be more reliable in vivo than UV-laser microscopy, is used to detect precursors in the biosynthetic pathway leading to Chl. The sensitivity and ability to distinguish different precursors with this system is compared to current methods. Furthermore, we report on optimization of the spectral scanning method with the aim to minimize the excitation light-evoked photo-conversion of the chlorophyll precursors.

U2 - 10.1039/b811774h

DO - 10.1039/b811774h

M3 - Journal article

C2 - 19247522

VL - 8

SP - 279

EP - 286

JO - Photochemical & Photobiological Sciences

JF - Photochemical & Photobiological Sciences

SN - 1474-905X

IS - 2

ER -

ID: 17265872