New insights into the structure and function of chemokine receptor:chemokine complexes from an experimental perspective
Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt
Chemokines are small soluble proteins that drive cell migration through the formation of concentration gradients. Chemokine binding to G protein-coupled chemokine receptors in the cell membrane activates intracellular signaling pathways and is a fundamental process involved in numerous physiological and pathophysiological functions. In the past few years, significant experimental developments have made it possible to characterize complexes between chemokine receptors and chemokines at a molecular level. Here, I review these developments from an experimental perspective, focusing on how the ability to express, purify, and stabilize receptor:chemokine complexes have made studies by X-ray crystallography, nuclear magnetic resonance, and other methods possible. I give examples of how these studies have advanced our understanding of the architecture of receptor:chemokine complexes as well as the mechanisms involved in complex formation. Finally, I discuss some of the many remaining questions and challenges that will require studies of more receptors and chemokines as well as further development of experimental methods.
Originalsprog | Engelsk |
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Tidsskrift | Journal of Leukocyte Biology |
Vol/bind | 107 |
Udgave nummer | 6 |
ISSN | 0741-5400 |
DOI | |
Status | Udgivet - 2020 |
ID: 237106490