Neutrophil pathways of inflammation characterize the blood transcriptomic signature of patients with psoriasis and cardiovascular disease
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Neutrophil pathways of inflammation characterize the blood transcriptomic signature of patients with psoriasis and cardiovascular disease. / Kvist‐hansen, Amanda; Kaiser, Hannah; Wang, Xing; Krakauer, Martin; Gørtz, Peter Michael; McCauley, Benjamin D.; Zachariae, Claus; Becker, Christine; Hansen, Peter Riis; Skov, Lone.
I: International Journal of Molecular Sciences, Bind 22, Nr. 19, 10818, 2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Neutrophil pathways of inflammation characterize the blood transcriptomic signature of patients with psoriasis and cardiovascular disease
AU - Kvist‐hansen, Amanda
AU - Kaiser, Hannah
AU - Wang, Xing
AU - Krakauer, Martin
AU - Gørtz, Peter Michael
AU - McCauley, Benjamin D.
AU - Zachariae, Claus
AU - Becker, Christine
AU - Hansen, Peter Riis
AU - Skov, Lone
N1 - Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021
Y1 - 2021
N2 - Background: Patients with psoriasis have an increased risk of atherosclerotic cardiovascular disease (CVD). The molecular mechanisms behind this connection are not fully understood, but the involvement of neutrophils have drawn attention as a shared inflammatory factor. Methods: RNA sequencing using the Illumina platform was performed on blood from 38 patients with mod-erate to severe psoriasis; approximately half had prior CVD. The neutrophil to lymphocyte ratio (NLR) was obtained from blood samples. Subclinical atherosclerosis was assessed by18F‐fluorode-oxyglucose positron emission tomography/computed tomography and ultrasound imaging. Tran-scriptomic analysis for differential expression and functional enrichment were performed, followed by correlation analyses of differentially expressed genes (DEGs), NLR and subclinical measurers of CVD. Results: 291 genes were differentially expressed between patients with psoriasis with and without CVD. These included 208 upregulated and 83 downregulated DEGs. Neutrophil degranu-lation was identified as the most significant process related to the upregulated DEGs. Genes for the neutrophil‐associated markers MPO, MMP9, LCN2, CEACAM1, CEACAM6 and CEACAM8 were identified as being of special interest and their mRNA levels correlated with NLR, high‐sensitive C‐ reactive protein and markers of subclinical CVD. Conclusions: Patients with psoriasis and CVD had an increased expression of genes related to neutrophil degranulation in their blood transcriptome compared with patients with psoriasis without CVD. NLR may be a potential biomarker of subclin-ical CVD in psoriasis.
AB - Background: Patients with psoriasis have an increased risk of atherosclerotic cardiovascular disease (CVD). The molecular mechanisms behind this connection are not fully understood, but the involvement of neutrophils have drawn attention as a shared inflammatory factor. Methods: RNA sequencing using the Illumina platform was performed on blood from 38 patients with mod-erate to severe psoriasis; approximately half had prior CVD. The neutrophil to lymphocyte ratio (NLR) was obtained from blood samples. Subclinical atherosclerosis was assessed by18F‐fluorode-oxyglucose positron emission tomography/computed tomography and ultrasound imaging. Tran-scriptomic analysis for differential expression and functional enrichment were performed, followed by correlation analyses of differentially expressed genes (DEGs), NLR and subclinical measurers of CVD. Results: 291 genes were differentially expressed between patients with psoriasis with and without CVD. These included 208 upregulated and 83 downregulated DEGs. Neutrophil degranu-lation was identified as the most significant process related to the upregulated DEGs. Genes for the neutrophil‐associated markers MPO, MMP9, LCN2, CEACAM1, CEACAM6 and CEACAM8 were identified as being of special interest and their mRNA levels correlated with NLR, high‐sensitive C‐ reactive protein and markers of subclinical CVD. Conclusions: Patients with psoriasis and CVD had an increased expression of genes related to neutrophil degranulation in their blood transcriptome compared with patients with psoriasis without CVD. NLR may be a potential biomarker of subclin-ical CVD in psoriasis.
KW - Cardiovascular disease
KW - Neutrophil to lymphocyte ratio
KW - Neutrophils
KW - Psoriasis
KW - RNA sequencing
KW - Subclinical atherosclerosis
KW - Transcriptome
U2 - 10.3390/ijms221910818
DO - 10.3390/ijms221910818
M3 - Journal article
C2 - 34639156
AN - SCOPUS:85116415217
VL - 22
JO - International Journal of Molecular Sciences (Online)
JF - International Journal of Molecular Sciences (Online)
SN - 1661-6596
IS - 19
M1 - 10818
ER -
ID: 284106769