Neurosphere based differentiation of human iPSC improves astrocyte differentiation
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- Neurosphere based differentiation of human iPSC improves astrocyte differentiation
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Neural progenitor cells (NPCs) derived from human induced pluripotent stem cells (iPSCs) are traditionally maintained and proliferated utilizing two-dimensional (2D) adherent monolayer culture systems. However, NPCs cultured using this system hardly reflect the intrinsic spatial development of brain tissue. In this study, we determined that culturing iPSC-derived NPCs as three-dimensional (3D) floating neurospheres resulted in increased expression of the neural progenitor cell (NPC) markers, PAX6 and NESTIN. Expansion of NPCs in 3D culture methods also resulted in a more homogenous PAX6 expression when compared to 2D culture methods. Furthermore, the 3D propagation method for NPCs resulted in a significant higher expression of the astrocyte markers GFAP and aquaporin 4 (AQP4) in the differentiated cells. Thus, our 3D propagation method could constitute a useful tool to promote NPC homogeneity and also to increase the differentiation potential of iPSC towards astrocytes.
Originalsprog | Engelsk |
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Artikelnummer | 4937689 |
Tidsskrift | Stem Cells International |
Vol/bind | 2016 |
Antal sider | 15 |
ISSN | 1687-966X |
DOI | |
Status | Udgivet - 2016 |
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