Near-Infrared In Vivo Whole-Body Fluorescence Imaging of PNA
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Near-Infrared In Vivo Whole-Body Fluorescence Imaging of PNA. / Lim, Ernest Wee Kiat; Brolin, Camilla; Nielsen, Peter E.
Peptide Nucleic Acids: Methods and Protocols. Humana Press, 2020. s. 251-260 (Methods in Molecular Biology, Bind 2105).Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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TY - CHAP
T1 - Near-Infrared In Vivo Whole-Body Fluorescence Imaging of PNA
AU - Lim, Ernest Wee Kiat
AU - Brolin, Camilla
AU - Nielsen, Peter E.
PY - 2020
Y1 - 2020
N2 - Using near-infrared fluorophore Alexa Fluor 680 labeled peptide nucleic acids (PNAs) the biodistribution of such antisense agents can be analyzed in real time in live mice using in vivo imaging. Using the fluorescence intensity emitted from the mouse at different time points following administration, the systemic distribution and organ accumulation of PNA can be tracked. In addition, an estimation of the body half-life of the compound can be obtained by the change in fluorescence intensity over time. With this technique, the distribution of compounds can be monitored real time, while reducing the number of animals and amount of compounds required.
AB - Using near-infrared fluorophore Alexa Fluor 680 labeled peptide nucleic acids (PNAs) the biodistribution of such antisense agents can be analyzed in real time in live mice using in vivo imaging. Using the fluorescence intensity emitted from the mouse at different time points following administration, the systemic distribution and organ accumulation of PNA can be tracked. In addition, an estimation of the body half-life of the compound can be obtained by the change in fluorescence intensity over time. With this technique, the distribution of compounds can be monitored real time, while reducing the number of animals and amount of compounds required.
KW - Biodistribution
KW - IVIS
KW - Tissue accumulation
KW - Whole-body in vivo bioimaging
U2 - 10.1007/978-1-0716-0243-0_16
DO - 10.1007/978-1-0716-0243-0_16
M3 - Book chapter
C2 - 32088876
AN - SCOPUS:85079912008
SN - 978-1-0716-0242-3
T3 - Methods in Molecular Biology
SP - 251
EP - 260
BT - Peptide Nucleic Acids
PB - Humana Press
ER -
ID: 239812468