Near-Infrared In Vivo Whole-Body Fluorescence Imaging of PNA

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

Using near-infrared fluorophore Alexa Fluor 680 labeled peptide nucleic acids (PNAs) the biodistribution of such antisense agents can be analyzed in real time in live mice using in vivo imaging. Using the fluorescence intensity emitted from the mouse at different time points following administration, the systemic distribution and organ accumulation of PNA can be tracked. In addition, an estimation of the body half-life of the compound can be obtained by the change in fluorescence intensity over time. With this technique, the distribution of compounds can be monitored real time, while reducing the number of animals and amount of compounds required.

OriginalsprogEngelsk
TitelPeptide Nucleic Acids : Methods and Protocols
Antal sider10
ForlagHumana Press
Publikationsdato2020
Sider251-260
ISBN (Trykt)978-1-0716-0242-3
ISBN (Elektronisk)978-1-0716-0243-0
DOI
StatusUdgivet - 2020
NavnMethods in Molecular Biology
Vol/bind2105
ISSN1064-3745

ID: 239812468