Multi-scale laser speckle contrast imaging of microcirculatory vasoreactivity
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Multi-scale laser speckle contrast imaging of microcirculatory vasoreactivity. / Lee, Blaire; Sosnovtseva, Olga; Sorensen, Charlotte M.; Postnov, Dmitry D.
I: Biomedical Optics Express, Bind 13, Nr. 4, 2022, s. 2312-2322.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Multi-scale laser speckle contrast imaging of microcirculatory vasoreactivity
AU - Lee, Blaire
AU - Sosnovtseva, Olga
AU - Sorensen, Charlotte M.
AU - Postnov, Dmitry D.
N1 - Publisher Copyright: © 2022 Optica Publishing Group.
PY - 2022
Y1 - 2022
N2 - Laser speckle contrast imaging is a robust and versatile blood flow imaging tool in basic and clinical research for its relatively simple construction and ease of customization. One of its key features is the scalability of the imaged field of view. With minimal changes to the system or analysis, laser speckle contrast imaging allows for high-resolution blood flow imaging through cranial windows or low-resolution perfusion visualization of perfusion over large areas, e.g. in human skin. We further utilize this feature and introduce a multi-scale laser speckle contrast imaging system, which we apply to study vasoreactivity in renal microcirculation. We combine high resolution (small field of view) to segment blood flow in individual vessels with low resolution (large field of view) to monitor global blood flow changes across the renal surface. Furthermore, we compare their performance when analyzing blood flow dynamics potentially associated with a single nephron and show that the previously published approaches, based on low-zoom imaging alone, provide inaccurate results in such applications.
AB - Laser speckle contrast imaging is a robust and versatile blood flow imaging tool in basic and clinical research for its relatively simple construction and ease of customization. One of its key features is the scalability of the imaged field of view. With minimal changes to the system or analysis, laser speckle contrast imaging allows for high-resolution blood flow imaging through cranial windows or low-resolution perfusion visualization of perfusion over large areas, e.g. in human skin. We further utilize this feature and introduce a multi-scale laser speckle contrast imaging system, which we apply to study vasoreactivity in renal microcirculation. We combine high resolution (small field of view) to segment blood flow in individual vessels with low resolution (large field of view) to monitor global blood flow changes across the renal surface. Furthermore, we compare their performance when analyzing blood flow dynamics potentially associated with a single nephron and show that the previously published approaches, based on low-zoom imaging alone, provide inaccurate results in such applications.
U2 - 10.1364/BOE.451014
DO - 10.1364/BOE.451014
M3 - Journal article
C2 - 35519248
AN - SCOPUS:85126932626
VL - 13
SP - 2312
EP - 2322
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 4
ER -
ID: 302555864