Multi-omics to predict changes during cold pressor test
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Background: The cold pressor test (CPT) is a widely used pain provocation test to investigate both pain tolerance and cardiovascular responses. We hypothesize, that performing multi-omic analyses during CPT gives the opportunity to home in on molecular mechanisms involved. Twenty-two females were phenotypically assessed before and after a CPT, and blood samples were taken. RNA-Sequencing, steroid profiling and untargeted metabolomics were performed. Each ‘omic level was analyzed separately at both single-feature and systems-level (principal component [PCA] and partial least squares [PLS] regression analysis) and all ‘omic levels were combined using an integrative multi-omics approach, all using the paired-sample design. Results: We showed that PCA was not able to discriminate time points, while PLS did significantly distinguish time points using metabolomics and/or transcriptomic data, but not using conventional physiological measures. Transcriptomic and metabolomic data revealed at feature-, systems- and integrative- level biologically relevant processes involved during CPT, e.g. lipid metabolism and stress response. Conclusion: Multi-omics strategies have a great potential in pain research, both at feature- and systems- level. Therefore, they should be exploited in intervention studies, such as pain provocation tests, to gain knowledge on the biological mechanisms involved in complex traits.
Originalsprog | Engelsk |
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Artikelnummer | 759 |
Tidsskrift | BMC Genomics |
Vol/bind | 23 |
Antal sider | 11 |
ISSN | 1471-2164 |
DOI | |
Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:
The work was funded by a grant from the Candys foundation ‘CEHEAD’ and EU-funded FP7 “EUROHEADPAIN” grant (no. 602633), both awarded to prof. Jes Olesen.
Publisher Copyright:
© 2022, The Author(s).
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