mRNA expression profile of prostaglandin D2 receptors in rat trigeminovascular system, and effect of prostaglandins in rat migraine models
Publikation: Bidrag til tidsskrift › Konferenceabstrakt i tidsskrift › Forskning › fagfællebedømt
Background: Prostaglandin D2 (PGD2) is a strong vasodilator
of extracerebral arteries, but causes only mild
headache in healthy volunteers.
Aims: 1.) To elucidate the mRNA expression profile of
DP1, DP2 receptors and PGD2 synthase (L-PGDS) in the
trigeminovascular system (TVS); and other pain processing
structures in rat brain; 2.) To study the effect of the DP1
receptor antagonist, MK-0524, on PGD2-induced vasodilation
of middle meningeal artery (MMA) in rat closed cranial
window (CCW) model; 3.) To investigate if an i.v.
infusion of prostaglandin (PG) mix, PGD2, PGE2 and PGI2
(iloprost), in awake rats activates nociceptive/neuronal
activation markers in the TVS.
Methods: qPCR, the genuine rat CCW model, and the
western blot technique were used to investigate the above
mentioned aims.
Results: mRNA for DP1, DP2 and L-PGDS was expressed
differentially in all examined tissues. The DP1 receptor was
highly expressed in trigeminal ganglion and dorsal rootganglion. MK-0524 significantly (62%, p<0.05) blocked
the PGD2- induced dilation of MMA. No increase in p-ERK
protein level was observed in the TVS after infusion of PG
mix in awake rats. Neuronal activation markers, cFOS and
EGR-1, were not changed in the trigeminal nucleus
caudalis.
Conclusions: PGD2 induced vasodilation of MMA is
mainly mediated by activation of DP1 receptors.
Furthermore, high expression of DP1 mRNA in TG and
DRG suggest that PGD2 might play a role in migraine
pathophysiology. However, infusion of PG mix in awake
rats did not activate nociceptive/neuronal markers in the
TVS.
Originalsprog | Engelsk |
---|---|
Artikelnummer | PO446 |
Tidsskrift | Cephalalgia |
Vol/bind | 35 |
Udgave nummer | suppl 6S |
Sider (fra-til) | 232-233 |
Antal sider | 2 |
ISSN | 0333-1024 |
DOI | |
Status | Udgivet - maj 2015 |
ID: 140493517