Mouse adhalin: primary structure and expression during late stages of muscle differentiation in vitro

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Standard

Mouse adhalin : primary structure and expression during late stages of muscle differentiation in vitro. / Liu, L; Vachon, P H; Kuang, W; Xu, Hong; Wewer, Ulla M.; Kylsten, P; Engvall, E.

I: Biochemical and Biophysical Research Communications, Bind 235, Nr. 1, 1997, s. 227-35.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Liu, L, Vachon, PH, Kuang, W, Xu, H, Wewer, UM, Kylsten, P & Engvall, E 1997, 'Mouse adhalin: primary structure and expression during late stages of muscle differentiation in vitro', Biochemical and Biophysical Research Communications, bind 235, nr. 1, s. 227-35. https://doi.org/10.1006/bbrc.1997.6757

APA

Liu, L., Vachon, P. H., Kuang, W., Xu, H., Wewer, U. M., Kylsten, P., & Engvall, E. (1997). Mouse adhalin: primary structure and expression during late stages of muscle differentiation in vitro. Biochemical and Biophysical Research Communications, 235(1), 227-35. https://doi.org/10.1006/bbrc.1997.6757

Vancouver

Liu L, Vachon PH, Kuang W, Xu H, Wewer UM, Kylsten P o.a. Mouse adhalin: primary structure and expression during late stages of muscle differentiation in vitro. Biochemical and Biophysical Research Communications. 1997;235(1):227-35. https://doi.org/10.1006/bbrc.1997.6757

Author

Liu, L ; Vachon, P H ; Kuang, W ; Xu, Hong ; Wewer, Ulla M. ; Kylsten, P ; Engvall, E. / Mouse adhalin : primary structure and expression during late stages of muscle differentiation in vitro. I: Biochemical and Biophysical Research Communications. 1997 ; Bind 235, Nr. 1. s. 227-35.

Bibtex

@article{64cbeec2f1d04d7585fe53f7c35eb4ea,
title = "Mouse adhalin: primary structure and expression during late stages of muscle differentiation in vitro",
abstract = "Adhalin, or alpha-sarcoglycan, is a 50-kDa glycoprotein that was originally characterized as a muscle membrane protein. The importance of adhalin is suggested by the diseases associated with its absence, notably the limb-girdle muscular dystrophies. However, the function of adhalin is unknown. To analyze the biological roles of adhalin, we cloned the mouse adhalin cDNA, raised peptide-specific antibodies to its cytoplasmic domain, and examined its expression and localization in vivo and in vitro. The mouse adhalin sequence was 80% identical to that of human, rabbit, and hamster. Adhalin was specifically expressed in striated muscle cells and their immediate precursors, and absent in many other cell types. Adhalin expression in embryonic mouse muscle was coincident with primary myogenesis. Its expression was found to be up-regulated at mRNA and protein levels during myogenic differentiation in vitro. The proper localization of adhalin to the muscle cell membrane was observed only in late stages of myotube maturation, coincident with the re-distribution of caveolin-3 and dystrophin. These data suggest that adhalin is highly specific for striated muscle and that it is linked with the formation of a fully functional muscle fiber.",
keywords = "Amino Acid Sequence, Animals, Caveolin 3, Caveolins, Cell Differentiation, Cloning, Molecular, Cytoskeletal Proteins, DNA, Complementary, Fluorescent Antibody Technique, Gene Expression Regulation, Developmental, Membrane Glycoproteins, Membrane Proteins, Mice, Mice, Inbred Strains, Molecular Sequence Data, Muscle Proteins, Muscle, Skeletal, Muscular Dystrophies, RNA, Messenger, Sarcoglycans, Sequence Homology, Amino Acid, Tumor Cells, Cultured, Up-Regulation",
author = "L Liu and Vachon, {P H} and W Kuang and Hong Xu and Wewer, {Ulla M.} and P Kylsten and E Engvall",
year = "1997",
doi = "10.1006/bbrc.1997.6757",
language = "English",
volume = "235",
pages = "227--35",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Mouse adhalin

T2 - primary structure and expression during late stages of muscle differentiation in vitro

AU - Liu, L

AU - Vachon, P H

AU - Kuang, W

AU - Xu, Hong

AU - Wewer, Ulla M.

AU - Kylsten, P

AU - Engvall, E

PY - 1997

Y1 - 1997

N2 - Adhalin, or alpha-sarcoglycan, is a 50-kDa glycoprotein that was originally characterized as a muscle membrane protein. The importance of adhalin is suggested by the diseases associated with its absence, notably the limb-girdle muscular dystrophies. However, the function of adhalin is unknown. To analyze the biological roles of adhalin, we cloned the mouse adhalin cDNA, raised peptide-specific antibodies to its cytoplasmic domain, and examined its expression and localization in vivo and in vitro. The mouse adhalin sequence was 80% identical to that of human, rabbit, and hamster. Adhalin was specifically expressed in striated muscle cells and their immediate precursors, and absent in many other cell types. Adhalin expression in embryonic mouse muscle was coincident with primary myogenesis. Its expression was found to be up-regulated at mRNA and protein levels during myogenic differentiation in vitro. The proper localization of adhalin to the muscle cell membrane was observed only in late stages of myotube maturation, coincident with the re-distribution of caveolin-3 and dystrophin. These data suggest that adhalin is highly specific for striated muscle and that it is linked with the formation of a fully functional muscle fiber.

AB - Adhalin, or alpha-sarcoglycan, is a 50-kDa glycoprotein that was originally characterized as a muscle membrane protein. The importance of adhalin is suggested by the diseases associated with its absence, notably the limb-girdle muscular dystrophies. However, the function of adhalin is unknown. To analyze the biological roles of adhalin, we cloned the mouse adhalin cDNA, raised peptide-specific antibodies to its cytoplasmic domain, and examined its expression and localization in vivo and in vitro. The mouse adhalin sequence was 80% identical to that of human, rabbit, and hamster. Adhalin was specifically expressed in striated muscle cells and their immediate precursors, and absent in many other cell types. Adhalin expression in embryonic mouse muscle was coincident with primary myogenesis. Its expression was found to be up-regulated at mRNA and protein levels during myogenic differentiation in vitro. The proper localization of adhalin to the muscle cell membrane was observed only in late stages of myotube maturation, coincident with the re-distribution of caveolin-3 and dystrophin. These data suggest that adhalin is highly specific for striated muscle and that it is linked with the formation of a fully functional muscle fiber.

KW - Amino Acid Sequence

KW - Animals

KW - Caveolin 3

KW - Caveolins

KW - Cell Differentiation

KW - Cloning, Molecular

KW - Cytoskeletal Proteins

KW - DNA, Complementary

KW - Fluorescent Antibody Technique

KW - Gene Expression Regulation, Developmental

KW - Membrane Glycoproteins

KW - Membrane Proteins

KW - Mice

KW - Mice, Inbred Strains

KW - Molecular Sequence Data

KW - Muscle Proteins

KW - Muscle, Skeletal

KW - Muscular Dystrophies

KW - RNA, Messenger

KW - Sarcoglycans

KW - Sequence Homology, Amino Acid

KW - Tumor Cells, Cultured

KW - Up-Regulation

U2 - 10.1006/bbrc.1997.6757

DO - 10.1006/bbrc.1997.6757

M3 - Journal article

C2 - 9196068

VL - 235

SP - 227

EP - 235

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -

ID: 38286817