Molecular Techniques for Genus and Species Determination of Fungi From Fresh and Paraffin-Embedded Formalin-Fixed Tissue in the Revised EORTC/MSGERC Definitions of Invasive Fungal Infection

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Molecular Techniques for Genus and Species Determination of Fungi From Fresh and Paraffin-Embedded Formalin-Fixed Tissue in the Revised EORTC/MSGERC Definitions of Invasive Fungal Infection. / Lockhart, Shawn R.; Bialek, Ralf; Kibbler, Christopher C.; Cuenca-Estrella, Manuel; Jensen, Henrik E.; Kontoyiannis, Dimitrios P.

I: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, Bind 72, Nr. 2, 2021, s. S109-S113.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Lockhart, SR, Bialek, R, Kibbler, CC, Cuenca-Estrella, M, Jensen, HE & Kontoyiannis, DP 2021, 'Molecular Techniques for Genus and Species Determination of Fungi From Fresh and Paraffin-Embedded Formalin-Fixed Tissue in the Revised EORTC/MSGERC Definitions of Invasive Fungal Infection', Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, bind 72, nr. 2, s. S109-S113. https://doi.org/10.1093/cid/ciaa1836

APA

Lockhart, S. R., Bialek, R., Kibbler, C. C., Cuenca-Estrella, M., Jensen, H. E., & Kontoyiannis, D. P. (2021). Molecular Techniques for Genus and Species Determination of Fungi From Fresh and Paraffin-Embedded Formalin-Fixed Tissue in the Revised EORTC/MSGERC Definitions of Invasive Fungal Infection. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 72(2), S109-S113. https://doi.org/10.1093/cid/ciaa1836

Vancouver

Lockhart SR, Bialek R, Kibbler CC, Cuenca-Estrella M, Jensen HE, Kontoyiannis DP. Molecular Techniques for Genus and Species Determination of Fungi From Fresh and Paraffin-Embedded Formalin-Fixed Tissue in the Revised EORTC/MSGERC Definitions of Invasive Fungal Infection. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 2021;72(2):S109-S113. https://doi.org/10.1093/cid/ciaa1836

Author

Lockhart, Shawn R. ; Bialek, Ralf ; Kibbler, Christopher C. ; Cuenca-Estrella, Manuel ; Jensen, Henrik E. ; Kontoyiannis, Dimitrios P. / Molecular Techniques for Genus and Species Determination of Fungi From Fresh and Paraffin-Embedded Formalin-Fixed Tissue in the Revised EORTC/MSGERC Definitions of Invasive Fungal Infection. I: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 2021 ; Bind 72, Nr. 2. s. S109-S113.

Bibtex

@article{723dffd9d5f444e995171bdecb436995,
title = "Molecular Techniques for Genus and Species Determination of Fungi From Fresh and Paraffin-Embedded Formalin-Fixed Tissue in the Revised EORTC/MSGERC Definitions of Invasive Fungal Infection",
abstract = "The EORTC/MSGERC have revised the definitions for proven, probable, and possible fungal diseases. The tissue diagnosis subcommittee was tasked with determining how and when species can be determined from tissue in the absence of culture. The subcommittee reached a consensus decision that polymerase chain reaction (PCR) from tissue, but not immunohistochemistry or in situ hybridization, can be used for genus or species determination under the new EORTC/MSGERC guidelines, but only when fungal elements are identified by histology. Fungal elements seen in tissue samples by histopathology and identified by PCR followed by sequencing should fulfill the definition of a proven fungal infection, identified to genus/species, even in the absence of culture. This summary discusses the issues that were deliberated by the subcommittee to reach the consensus decision and outlines the criteria a laboratory should follow in order to produce data that meet the EORTC/MSGERC definitions.",
keywords = "EORTC/MSG, FFPE, formalin-fixed paraffin-embedded tissue, immunohistochemistry, tissue diagnosis",
author = "Lockhart, {Shawn R.} and Ralf Bialek and Kibbler, {Christopher C.} and Manuel Cuenca-Estrella and Jensen, {Henrik E.} and Kontoyiannis, {Dimitrios P.}",
year = "2021",
doi = "10.1093/cid/ciaa1836",
language = "English",
volume = "72",
pages = "S109--S113",
journal = "Clinical Infectious Diseases",
issn = "1058-4838",
publisher = "Oxford University Press",
number = "2",

}

RIS

TY - JOUR

T1 - Molecular Techniques for Genus and Species Determination of Fungi From Fresh and Paraffin-Embedded Formalin-Fixed Tissue in the Revised EORTC/MSGERC Definitions of Invasive Fungal Infection

AU - Lockhart, Shawn R.

AU - Bialek, Ralf

AU - Kibbler, Christopher C.

AU - Cuenca-Estrella, Manuel

AU - Jensen, Henrik E.

AU - Kontoyiannis, Dimitrios P.

PY - 2021

Y1 - 2021

N2 - The EORTC/MSGERC have revised the definitions for proven, probable, and possible fungal diseases. The tissue diagnosis subcommittee was tasked with determining how and when species can be determined from tissue in the absence of culture. The subcommittee reached a consensus decision that polymerase chain reaction (PCR) from tissue, but not immunohistochemistry or in situ hybridization, can be used for genus or species determination under the new EORTC/MSGERC guidelines, but only when fungal elements are identified by histology. Fungal elements seen in tissue samples by histopathology and identified by PCR followed by sequencing should fulfill the definition of a proven fungal infection, identified to genus/species, even in the absence of culture. This summary discusses the issues that were deliberated by the subcommittee to reach the consensus decision and outlines the criteria a laboratory should follow in order to produce data that meet the EORTC/MSGERC definitions.

AB - The EORTC/MSGERC have revised the definitions for proven, probable, and possible fungal diseases. The tissue diagnosis subcommittee was tasked with determining how and when species can be determined from tissue in the absence of culture. The subcommittee reached a consensus decision that polymerase chain reaction (PCR) from tissue, but not immunohistochemistry or in situ hybridization, can be used for genus or species determination under the new EORTC/MSGERC guidelines, but only when fungal elements are identified by histology. Fungal elements seen in tissue samples by histopathology and identified by PCR followed by sequencing should fulfill the definition of a proven fungal infection, identified to genus/species, even in the absence of culture. This summary discusses the issues that were deliberated by the subcommittee to reach the consensus decision and outlines the criteria a laboratory should follow in order to produce data that meet the EORTC/MSGERC definitions.

KW - EORTC/MSG

KW - FFPE

KW - formalin-fixed paraffin-embedded tissue

KW - immunohistochemistry

KW - tissue diagnosis

U2 - 10.1093/cid/ciaa1836

DO - 10.1093/cid/ciaa1836

M3 - Journal article

C2 - 33709128

AN - SCOPUS:85102823494

VL - 72

SP - S109-S113

JO - Clinical Infectious Diseases

JF - Clinical Infectious Diseases

SN - 1058-4838

IS - 2

ER -

ID: 259101147