Microsatellite Primers for Parkia biglobosa (Fabaceae: Mimosoideae) Reveal that a Single Plant Sires All Seeds Per Pod

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Standard

Microsatellite Primers for Parkia biglobosa (Fabaceae: Mimosoideae) Reveal that a Single Plant Sires All Seeds Per Pod. / Lassen, Kristin Marie; Kjær, Erik Dahl; Ouédraogo, Moussa; Nielsen, Lene Rostgaard.

I: Applications in Plant Sciences, Bind 2, Nr. 6, 1400024, 2014.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Lassen, KM, Kjær, ED, Ouédraogo, M & Nielsen, LR 2014, 'Microsatellite Primers for Parkia biglobosa (Fabaceae: Mimosoideae) Reveal that a Single Plant Sires All Seeds Per Pod', Applications in Plant Sciences, bind 2, nr. 6, 1400024. https://doi.org/10.3732/apps.1400024

APA

Lassen, K. M., Kjær, E. D., Ouédraogo, M., & Nielsen, L. R. (2014). Microsatellite Primers for Parkia biglobosa (Fabaceae: Mimosoideae) Reveal that a Single Plant Sires All Seeds Per Pod. Applications in Plant Sciences, 2(6), [1400024]. https://doi.org/10.3732/apps.1400024

Vancouver

Lassen KM, Kjær ED, Ouédraogo M, Nielsen LR. Microsatellite Primers for Parkia biglobosa (Fabaceae: Mimosoideae) Reveal that a Single Plant Sires All Seeds Per Pod. Applications in Plant Sciences. 2014;2(6). 1400024. https://doi.org/10.3732/apps.1400024

Author

Lassen, Kristin Marie ; Kjær, Erik Dahl ; Ouédraogo, Moussa ; Nielsen, Lene Rostgaard. / Microsatellite Primers for Parkia biglobosa (Fabaceae: Mimosoideae) Reveal that a Single Plant Sires All Seeds Per Pod. I: Applications in Plant Sciences. 2014 ; Bind 2, Nr. 6.

Bibtex

@article{ad8d7f58b6964762bc3243e027b7160b,
title = "Microsatellite Primers for Parkia biglobosa (Fabaceae: Mimosoideae) Reveal that a Single Plant Sires All Seeds Per Pod",
abstract = "Premise of the study: Microsatellite primers were developed for an indigenous fruit tree, Parkia biglobosa, as a tool to study reproductive biology and population structure. Here we use the primers to determine the number of fathers per pod. Methods and Results: Microsatellite loci were enriched in a genomic sample and isolated using pyrosequencing. Eleven primer pairs were characterized in two populations of P. biglobosa in Burkina Faso (each with 40 trees). The number of alleles per locus ranged from eight to 15, and one locus had null alleles. We genotyped seeds from 24 open-pollinated pods. The genotypic profiles of seeds per pod suggest that all seeds are outcrossed and that only one pollen donor sires all ovules in a single fruit. Conclusions: Ten microsatellite markers were highly polymorphic. All seeds per pod of P. biglobosa were full siblings. The markers will be useful for reproductive and population genetic studies.",
author = "Lassen, {Kristin Marie} and Kj{\ae}r, {Erik Dahl} and Moussa Ou{\'e}draogo and Nielsen, {Lene Rostgaard}",
year = "2014",
doi = "10.3732/apps.1400024",
language = "English",
volume = "2",
journal = "Applications in Plant Sciences",
issn = "2168-0450",
publisher = "Botanical Society of America",
number = "6",

}

RIS

TY - JOUR

T1 - Microsatellite Primers for Parkia biglobosa (Fabaceae: Mimosoideae) Reveal that a Single Plant Sires All Seeds Per Pod

AU - Lassen, Kristin Marie

AU - Kjær, Erik Dahl

AU - Ouédraogo, Moussa

AU - Nielsen, Lene Rostgaard

PY - 2014

Y1 - 2014

N2 - Premise of the study: Microsatellite primers were developed for an indigenous fruit tree, Parkia biglobosa, as a tool to study reproductive biology and population structure. Here we use the primers to determine the number of fathers per pod. Methods and Results: Microsatellite loci were enriched in a genomic sample and isolated using pyrosequencing. Eleven primer pairs were characterized in two populations of P. biglobosa in Burkina Faso (each with 40 trees). The number of alleles per locus ranged from eight to 15, and one locus had null alleles. We genotyped seeds from 24 open-pollinated pods. The genotypic profiles of seeds per pod suggest that all seeds are outcrossed and that only one pollen donor sires all ovules in a single fruit. Conclusions: Ten microsatellite markers were highly polymorphic. All seeds per pod of P. biglobosa were full siblings. The markers will be useful for reproductive and population genetic studies.

AB - Premise of the study: Microsatellite primers were developed for an indigenous fruit tree, Parkia biglobosa, as a tool to study reproductive biology and population structure. Here we use the primers to determine the number of fathers per pod. Methods and Results: Microsatellite loci were enriched in a genomic sample and isolated using pyrosequencing. Eleven primer pairs were characterized in two populations of P. biglobosa in Burkina Faso (each with 40 trees). The number of alleles per locus ranged from eight to 15, and one locus had null alleles. We genotyped seeds from 24 open-pollinated pods. The genotypic profiles of seeds per pod suggest that all seeds are outcrossed and that only one pollen donor sires all ovules in a single fruit. Conclusions: Ten microsatellite markers were highly polymorphic. All seeds per pod of P. biglobosa were full siblings. The markers will be useful for reproductive and population genetic studies.

U2 - 10.3732/apps.1400024

DO - 10.3732/apps.1400024

M3 - Journal article

C2 - 25202634

VL - 2

JO - Applications in Plant Sciences

JF - Applications in Plant Sciences

SN - 2168-0450

IS - 6

M1 - 1400024

ER -

ID: 117480307