MicroRNA miR-125b induces senescence in human melanoma cells

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

MicroRNA miR-125b induces senescence in human melanoma cells. / Glud, Martin; Manfé, Valentina; Biskup, Edyta; Holst, Line; Dirksen, Anne Marie Ahlburg; Hastrup, Nina; Nielsen, Finn C; Drzewiecki, Krzysztof T; Gniadecki, Robert.

I: Melanoma Research, Bind 21, Nr. 3, 01.06.2011, s. 253-6.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Glud, M, Manfé, V, Biskup, E, Holst, L, Dirksen, AMA, Hastrup, N, Nielsen, FC, Drzewiecki, KT & Gniadecki, R 2011, 'MicroRNA miR-125b induces senescence in human melanoma cells', Melanoma Research, bind 21, nr. 3, s. 253-6. https://doi.org/10.1097/CMR.0b013e328345333b, https://doi.org/10.1097/CMR.0b013e328345333b

APA

Glud, M., Manfé, V., Biskup, E., Holst, L., Dirksen, A. M. A., Hastrup, N., Nielsen, F. C., Drzewiecki, K. T., & Gniadecki, R. (2011). MicroRNA miR-125b induces senescence in human melanoma cells. Melanoma Research, 21(3), 253-6. https://doi.org/10.1097/CMR.0b013e328345333b, https://doi.org/10.1097/CMR.0b013e328345333b

Vancouver

Glud M, Manfé V, Biskup E, Holst L, Dirksen AMA, Hastrup N o.a. MicroRNA miR-125b induces senescence in human melanoma cells. Melanoma Research. 2011 jun. 1;21(3):253-6. https://doi.org/10.1097/CMR.0b013e328345333b, https://doi.org/10.1097/CMR.0b013e328345333b

Author

Glud, Martin ; Manfé, Valentina ; Biskup, Edyta ; Holst, Line ; Dirksen, Anne Marie Ahlburg ; Hastrup, Nina ; Nielsen, Finn C ; Drzewiecki, Krzysztof T ; Gniadecki, Robert. / MicroRNA miR-125b induces senescence in human melanoma cells. I: Melanoma Research. 2011 ; Bind 21, Nr. 3. s. 253-6.

Bibtex

@article{260b0f70585a4947b44d5c59dfd53045,

title = "MicroRNA miR-125b induces senescence in human melanoma cells",

abstract = "MicroRNAs (miRNAs) are small noncoding RNA molecules involved in gene regulation. Aberrant expression of miRNA has been associated with the development or progression of several diseases, including cancer. In a previous study, we found that the expression of miRNA-125b (miR-125b) was two-fold lower in malignant melanoma producing lymph node micrometastases than in nonmetastasizing tumors. To get further insight into the functional role of miR-125b, we assessed whether its overexpression or silencing affects apoptosis, proliferation, or senescence in melanoma cell lines. We showed that overexpression of miR-125b induced typical senescent cell morphology, including increased cytoplasmatic/nucleus ratio and intensive cytoplasmatic {\ss}-galactosidase expression. In contrast, inhibition of miR-125b resulted in 30-35% decreased levels of spontaneous apoptosis. We propose that downregulation of miR-125b in an early cutaneous malignant melanoma can contribute to the increased metastatic capability of this tumor.",

author = "Martin Glud and Valentina Manf{\'e} and Edyta Biskup and Line Holst and Dirksen, {Anne Marie Ahlburg} and Nina Hastrup and Nielsen, {Finn C} and Drzewiecki, {Krzysztof T} and Robert Gniadecki",

year = "2011",

month = jun,

day = "1",

doi = "10.1097/CMR.0b013e328345333b",

language = "English",

volume = "21",

pages = "253--6",

journal = "Melanoma Research",

issn = "0960-8931",

publisher = "Lippincott Williams & Wilkins, Ltd.",

number = "3",

}

RIS

TY - JOUR

T1 - MicroRNA miR-125b induces senescence in human melanoma cells

AU - Glud, Martin

AU - Manfé, Valentina

AU - Biskup, Edyta

AU - Holst, Line

AU - Dirksen, Anne Marie Ahlburg

AU - Hastrup, Nina

AU - Nielsen, Finn C

AU - Drzewiecki, Krzysztof T

AU - Gniadecki, Robert

PY - 2011/6/1

Y1 - 2011/6/1

N2 - MicroRNAs (miRNAs) are small noncoding RNA molecules involved in gene regulation. Aberrant expression of miRNA has been associated with the development or progression of several diseases, including cancer. In a previous study, we found that the expression of miRNA-125b (miR-125b) was two-fold lower in malignant melanoma producing lymph node micrometastases than in nonmetastasizing tumors. To get further insight into the functional role of miR-125b, we assessed whether its overexpression or silencing affects apoptosis, proliferation, or senescence in melanoma cell lines. We showed that overexpression of miR-125b induced typical senescent cell morphology, including increased cytoplasmatic/nucleus ratio and intensive cytoplasmatic ß-galactosidase expression. In contrast, inhibition of miR-125b resulted in 30-35% decreased levels of spontaneous apoptosis. We propose that downregulation of miR-125b in an early cutaneous malignant melanoma can contribute to the increased metastatic capability of this tumor.

AB - MicroRNAs (miRNAs) are small noncoding RNA molecules involved in gene regulation. Aberrant expression of miRNA has been associated with the development or progression of several diseases, including cancer. In a previous study, we found that the expression of miRNA-125b (miR-125b) was two-fold lower in malignant melanoma producing lymph node micrometastases than in nonmetastasizing tumors. To get further insight into the functional role of miR-125b, we assessed whether its overexpression or silencing affects apoptosis, proliferation, or senescence in melanoma cell lines. We showed that overexpression of miR-125b induced typical senescent cell morphology, including increased cytoplasmatic/nucleus ratio and intensive cytoplasmatic ß-galactosidase expression. In contrast, inhibition of miR-125b resulted in 30-35% decreased levels of spontaneous apoptosis. We propose that downregulation of miR-125b in an early cutaneous malignant melanoma can contribute to the increased metastatic capability of this tumor.

U2 - 10.1097/CMR.0b013e328345333b

DO - 10.1097/CMR.0b013e328345333b

M3 - Journal article

C2 - 21460750

VL - 21

SP - 253

EP - 256

JO - Melanoma Research

JF - Melanoma Research

SN - 0960-8931

IS - 3

ER -

ID: 34073547