Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
Fusion of vesicles with the plasma membrane and liberation of their contents is a multistep process involving several proteins. Correctly assigning the role of specific proteins and reactions in this cascade requires a measurement method with high temporal resolution. Patch-clamp recordings of cell membrane capacitance in combination with calcium measurements, calcium uncaging, and carbon-fiber amperometry allow for the accurate determination of vesicle pool sizes, their fusion kinetics, and their secreted oxidizable content. Here, we will describe this method in a model system for neurosecretion, the adrenal chromaffin cells, which secrete adrenaline.
Originalsprog | Engelsk |
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Titel | Methods in Molecular Biology |
Redaktører | Florence Niedergang, Nicolas Vitale, Stéphane Gasman |
Antal sider | 19 |
Udgivelsessted | New Yorj |
Forlag | Humana Press |
Publikationsdato | 2021 |
Udgave | 1 |
Sider | 233-251 |
ISBN (Trykt) | 978-1-0716-1043-5 |
ISBN (Elektronisk) | 978-1-0716-1044-2 |
DOI | |
Status | Udgivet - 2021 |
Navn | Methods in Molecular Biology |
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Vol/bind | 2233 |
ISSN | 1064-3745 |
ID: 258774298