Mapping of linear B-cell epitopes on the major core protein p24 of human immunodeficiency virus type 1 (HIV-1)
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Mapping of linear B-cell epitopes on the major core protein p24 of human immunodeficiency virus type 1 (HIV-1). / Kusk, P; Bugge, T H; Lindhardt, B O; Hulgaard, E F; Holmbäck, K.
I: AIDS Research and Human Retroviruses, Bind 8, Nr. 10, 10.1992, s. 1789-94.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Mapping of linear B-cell epitopes on the major core protein p24 of human immunodeficiency virus type 1 (HIV-1)
AU - Kusk, P
AU - Bugge, T H
AU - Lindhardt, B O
AU - Hulgaard, E F
AU - Holmbäck, K
PY - 1992/10
Y1 - 1992/10
N2 - Three murine monoclonal antibodies (MAbs) F5-2, F5-4, and F5-16 defining three different epitopes on the major core protein p24 of the human immunodeficiency virus type 1 (HIV-1) were epitope mapped using a random fragment expression library representing the p17- and p24-encoding part of the gag open reading frame. F5-2 defined an epitope within amino acids (aa) 14-23 at the N-terminus of p24, and F5-4 defined an epitope within aa 153-174 in the C-terminus of p24. F5-16 did not recognize any of the fusion proteins produced by the expression library indicating that this MAb defines a true conformational epitope on p24. Since the N-terminus of p24 has been reported to be immunosilent in humans, 356 HIV-1 antibody-positive serum samples were tested for reactivity against the region of p24 defined by F5-2. More than one third of the samples recognized this region indicating that it is immunoreactive and, further, the presence of antibodies against this region was associated with a reduced CD4 cell count.
AB - Three murine monoclonal antibodies (MAbs) F5-2, F5-4, and F5-16 defining three different epitopes on the major core protein p24 of the human immunodeficiency virus type 1 (HIV-1) were epitope mapped using a random fragment expression library representing the p17- and p24-encoding part of the gag open reading frame. F5-2 defined an epitope within amino acids (aa) 14-23 at the N-terminus of p24, and F5-4 defined an epitope within aa 153-174 in the C-terminus of p24. F5-16 did not recognize any of the fusion proteins produced by the expression library indicating that this MAb defines a true conformational epitope on p24. Since the N-terminus of p24 has been reported to be immunosilent in humans, 356 HIV-1 antibody-positive serum samples were tested for reactivity against the region of p24 defined by F5-2. More than one third of the samples recognized this region indicating that it is immunoreactive and, further, the presence of antibodies against this region was associated with a reduced CD4 cell count.
KW - Amino Acid Sequence
KW - Animals
KW - Antibodies, Monoclonal
KW - B-Lymphocytes/immunology
KW - Base Sequence
KW - DNA, Viral/genetics
KW - Epitopes/genetics
KW - HIV Antibodies
KW - HIV Core Protein p24/genetics
KW - HIV-1/genetics
KW - Humans
KW - In Vitro Techniques
KW - Male
KW - Mice
KW - Molecular Sequence Data
U2 - 10.1089/aid.1992.8.1789
DO - 10.1089/aid.1992.8.1789
M3 - Journal article
C2 - 1280956
VL - 8
SP - 1789
EP - 1794
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
SN - 0889-2229
IS - 10
ER -
ID: 201190685