Mapping Cellular Coordinates through Advances in Spatial Transcriptomics Technology
Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt
Dokumenter
- KSMCB043-07-01
Forlagets udgivne version, 1,23 MB, PDF-dokument
Complex cell-to-cell communication underlies the basic processes essential for homeostasis in the given tissue architecture. Obtaining quantitative gene-expression of cells in their native context has significantly advanced through single-cell RNA sequencing technologies along with mechanical and enzymatic tissue manipulation. This approach, however, is largely reliant on the physical dissociation of individual cells from the tissue, thus, resulting in a library with unaccounted positional information. To overcome this, positional information can be obtained by integrating imaging and positional barcoding. Collectively, spatial transcriptomics strategies provide tissue architecture-dependent as well as position-dependent cellular functions. This review discusses the current technologies for spatial transcriptomics ranging from the methods combining mechanical dissociation and single-cell RNA sequencing to computational spatial re-mapping.
Originalsprog | Engelsk |
---|---|
Tidsskrift | Molecules and Cells |
Vol/bind | 43 |
Udgave nummer | 7 |
Sider (fra-til) | 591-599 |
Antal sider | 9 |
ISSN | 1016-8478 |
DOI | |
Status | Udgivet - 2020 |
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ID: 247158723