Mapping Cellular Coordinates through Advances in Spatial Transcriptomics Technology

Publikation: Bidrag til tidsskriftReviewForskningfagfællebedømt

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Complex cell-to-cell communication underlies the basic processes essential for homeostasis in the given tissue architecture. Obtaining quantitative gene-expression of cells in their native context has significantly advanced through single-cell RNA sequencing technologies along with mechanical and enzymatic tissue manipulation. This approach, however, is largely reliant on the physical dissociation of individual cells from the tissue, thus, resulting in a library with unaccounted positional information. To overcome this, positional information can be obtained by integrating imaging and positional barcoding. Collectively, spatial transcriptomics strategies provide tissue architecture-dependent as well as position-dependent cellular functions. This review discusses the current technologies for spatial transcriptomics ranging from the methods combining mechanical dissociation and single-cell RNA sequencing to computational spatial re-mapping.

OriginalsprogEngelsk
TidsskriftMolecules and Cells
Vol/bind43
Udgave nummer7
Sider (fra-til)591-599
Antal sider9
ISSN1016-8478
DOI
StatusUdgivet - 2020

ID: 247158723