Low-voltage electromembrane extraction of basic drugs from biological samples
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
The present work has for the first time demonstrated electromembrane extraction (EME) at voltages obtainable by common batteries. Five basic drugs were extracted from acidified aqueous sample solutions, across a supported liquid membrane (SLM) consisting of 1-isopropyl-4-nitrobenzene impregnated in the walls of a hollow fiber, and into an acidified aqueous acceptor solution present inside the lumen of the hollow fiber with potential differences of 1-10 V applied over the SLM. Extractions from 1 ml standard solutions prepared in 10 mM HCl for 5 min and with a potential of 10 V demonstrated analyte recoveries of 50-93% in 25 μl of 10 mM HCl as acceptor solution. This corresponds to enrichment factors of 20-37. Similar results were obtained with a common 9 V battery as power supply. Recoveries from low-voltage EME on human plasma, urine, and breast milk diluted with acetate buffer (pH 4) demonstrated recoveries in the range of 37-55% after 5 min of extraction. Excellent selectivity was demonstrated as no interfering peaks were detected. Standard curves in the range of 0.0625-0.625 μg/ml demonstrated correlation coefficients of 0.994-0.999. Extraction recoveries from human plasma, urine or breast milk were not found to be sensitive towards individual variations. The results show that low-voltage EME has a future potential as a simple, selective, and time-efficient sample preparation technique of biological fluids.
Originalsprog | Engelsk |
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Tidsskrift | Journal of Chromatography A |
Vol/bind | 1180 |
Udgave nummer | 1-2 |
Sider (fra-til) | 1-9 |
Antal sider | 9 |
ISSN | 0021-9673 |
DOI | |
Status | Udgivet - 8 feb. 2008 |
ID: 231650656