Loss of PICH Results in Chromosomal Instability, p53 Activation, and Embryonic Lethality
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Loss of PICH Results in Chromosomal Instability, p53 Activation, and Embryonic Lethality. / Albers, Eliene; Sbroggiò, Mauro; Pladevall-Morera, David; Bizard, Anna H.; Avram, Alexandra; Gonzalez, Patricia; Martin-Gonzalez, Javier; Hickson, Ian D.; Lopez-Contreras, Andres J.
I: Cell Reports, Bind 24, Nr. 12, 2018, s. 3274-3284.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Loss of PICH Results in Chromosomal Instability, p53 Activation, and Embryonic Lethality
AU - Albers, Eliene
AU - Sbroggiò, Mauro
AU - Pladevall-Morera, David
AU - Bizard, Anna H.
AU - Avram, Alexandra
AU - Gonzalez, Patricia
AU - Martin-Gonzalez, Javier
AU - Hickson, Ian D.
AU - Lopez-Contreras, Andres J.
PY - 2018
Y1 - 2018
N2 - PICH is a DNA translocase necessary for the resolution of ultrafine anaphase DNA bridges and to ensure the fidelity of chromosomal segregation. Here, we report the generation of an animal model deficient for PICH that allowed us to investigate its physiological relevance. Pich KO mice lose viability during embryonic development due to a global accumulation of DNA damage. However, despite the presence of chromosomal instability, extensive p53 activation, and increased apoptosis throughout the embryo, Pich KO embryos survive until day 12.5 of embryonic development. The absence of p53 failed to improve the viability of the Pich KO embryos, suggesting that the observed developmental defects are not solely due to p53-induced apoptosis. Moreover, Pich-deficient mouse embryonic fibroblasts exhibit chromosomal instability and are resistant to RASV12/E1A-induced transformation. Overall, our data indicate that PICH is essential to preserve chromosomal integrity in rapidly proliferating cells and is therefore critical during embryonic development and tumorigenesis. Albers et al. show that PICH is essential for mouse embryonic development and that PICH deficiency limits oncogenic-induced cellular transformation. These findings suggest that PICH activity is critical during events requiring rapid cell proliferation such as embryonic development and tumorigenesis.
AB - PICH is a DNA translocase necessary for the resolution of ultrafine anaphase DNA bridges and to ensure the fidelity of chromosomal segregation. Here, we report the generation of an animal model deficient for PICH that allowed us to investigate its physiological relevance. Pich KO mice lose viability during embryonic development due to a global accumulation of DNA damage. However, despite the presence of chromosomal instability, extensive p53 activation, and increased apoptosis throughout the embryo, Pich KO embryos survive until day 12.5 of embryonic development. The absence of p53 failed to improve the viability of the Pich KO embryos, suggesting that the observed developmental defects are not solely due to p53-induced apoptosis. Moreover, Pich-deficient mouse embryonic fibroblasts exhibit chromosomal instability and are resistant to RASV12/E1A-induced transformation. Overall, our data indicate that PICH is essential to preserve chromosomal integrity in rapidly proliferating cells and is therefore critical during embryonic development and tumorigenesis. Albers et al. show that PICH is essential for mouse embryonic development and that PICH deficiency limits oncogenic-induced cellular transformation. These findings suggest that PICH activity is critical during events requiring rapid cell proliferation such as embryonic development and tumorigenesis.
KW - DNA damage
KW - Ercc6l
KW - genomic instability
KW - Pich
KW - UFBs
KW - ultrafine anaphase DNA bridges
KW - X chromosome inactivation
U2 - 10.1016/j.celrep.2018.08.071
DO - 10.1016/j.celrep.2018.08.071
M3 - Journal article
C2 - 30232008
AN - SCOPUS:85053129944
VL - 24
SP - 3274
EP - 3284
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 12
ER -
ID: 209673105