Long-term carriage and evolution of VREfmLong-term carriage and evolution of vancomycin-resistant Enterococcus faecium: A genomic study on consecutive isolates
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Objectives
To determine if vancomycin-resistant Enterococcus faecium (VREfm) carriers carry the same VREfm clone after a minimum follow-up of 365 days. For those carrying the same clone, we investigated the genomic evolution per year per genome.
Methods
We used WGS results to assign VREfm clones to each isolate and determine clone shifts. Finally, we calculated distance in core-genome MLST alleles, and the number of SNPs between consecutive VREfm isolates from patients carrying the same VREfm clone.
Results
In total, 44.2% of patients carried the same VREfm clone, and the genomic evolution was 1.8 alleles and 2.6 SNPs per genome per year.
Conclusions
In our population of long-term carriers, we calculated a molecular clock of 2.6 SNPs.
To determine if vancomycin-resistant Enterococcus faecium (VREfm) carriers carry the same VREfm clone after a minimum follow-up of 365 days. For those carrying the same clone, we investigated the genomic evolution per year per genome.
Methods
We used WGS results to assign VREfm clones to each isolate and determine clone shifts. Finally, we calculated distance in core-genome MLST alleles, and the number of SNPs between consecutive VREfm isolates from patients carrying the same VREfm clone.
Results
In total, 44.2% of patients carried the same VREfm clone, and the genomic evolution was 1.8 alleles and 2.6 SNPs per genome per year.
Conclusions
In our population of long-term carriers, we calculated a molecular clock of 2.6 SNPs.
| Originalsprog | Engelsk |
|---|---|
| Artikelnummer | dlad153 |
| Tidsskrift | JAC-Antimicrobial Resistance |
| Vol/bind | 6 |
| Udgave nummer | 1 |
| Antal sider | 4 |
| ISSN | 2632-1823 |
| DOI | |
| Status | Udgivet - 2024 |
Bibliografisk note
Funding Information:
The WGS was internally funded as a part of the routine work. The project was further funded by the Scandinavian Society for Antimicrobial Chemotherapy (SSAC) Foundation, the Regions' Medical and Treatment Foundation and the Danmarks Frie Forskningsfond.
Publisher Copyright:
© 2023 The Author(s). Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy.
ID: 381057968