Localization and biosynthesis of polyamines in insulin-producing cells

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Standard

Localization and biosynthesis of polyamines in insulin-producing cells. / Hougaard, D M; Larsson, L I; Nielsen, Jens Høiriis.

I: Biochemical Journal, Bind 238, Nr. 1, 15.08.1986, s. 43-7.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Hougaard, DM, Larsson, LI & Nielsen, JH 1986, 'Localization and biosynthesis of polyamines in insulin-producing cells', Biochemical Journal, bind 238, nr. 1, s. 43-7.

APA

Hougaard, D. M., Larsson, L. I., & Nielsen, J. H. (1986). Localization and biosynthesis of polyamines in insulin-producing cells. Biochemical Journal, 238(1), 43-7.

Vancouver

Hougaard DM, Larsson LI, Nielsen JH. Localization and biosynthesis of polyamines in insulin-producing cells. Biochemical Journal. 1986 aug 15;238(1):43-7.

Author

Hougaard, D M ; Larsson, L I ; Nielsen, Jens Høiriis. / Localization and biosynthesis of polyamines in insulin-producing cells. I: Biochemical Journal. 1986 ; Bind 238, Nr. 1. s. 43-7.

Bibtex

@article{a2b618eefd884d279f45735dc56db707,
title = "Localization and biosynthesis of polyamines in insulin-producing cells",
abstract = "Two recently developed fluorescence cytochemical methods, specific for spermidine and spermine, were used to localize polyamines in the endocrine pancreas. The polyamines were restricted to the insulin-producing beta-cells and were mainly associated with the secretory granules. Chemical polyamine determinations carried out on isolated rat and mouse pancreatic islets revealed large amounts of polyamines. Compared with extracts of whole pancreas, the islets contained very high concentrations of spermine relative to spermidine. Biosynthesis of polyamines from [3H]ornithine or from [3H]putrescine in isolated islets was significantly stimulated at high glucose concentrations. Moreover, significant incorporation of label from [3H]putrescine was also detected in gamma-aminobutyric acid. This incorporation, however, was not stimulated by high glucose. Possible roles for polyamines associated with the secretory granules in insulin-producing cells are discussed.",
keywords = "Animals, Cells, Cultured, Glucose, Immunoenzyme Techniques, Insulin, Islets of Langerhans, Mice, Ornithine, Putrescine, Rats, Rats, Inbred Strains, Spermidine, Spermine",
author = "Hougaard, {D M} and Larsson, {L I} and Nielsen, {Jens H{\o}iriis}",
year = "1986",
month = aug,
day = "15",
language = "English",
volume = "238",
pages = "43--7",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",
number = "1",

}

RIS

TY - JOUR

T1 - Localization and biosynthesis of polyamines in insulin-producing cells

AU - Hougaard, D M

AU - Larsson, L I

AU - Nielsen, Jens Høiriis

PY - 1986/8/15

Y1 - 1986/8/15

N2 - Two recently developed fluorescence cytochemical methods, specific for spermidine and spermine, were used to localize polyamines in the endocrine pancreas. The polyamines were restricted to the insulin-producing beta-cells and were mainly associated with the secretory granules. Chemical polyamine determinations carried out on isolated rat and mouse pancreatic islets revealed large amounts of polyamines. Compared with extracts of whole pancreas, the islets contained very high concentrations of spermine relative to spermidine. Biosynthesis of polyamines from [3H]ornithine or from [3H]putrescine in isolated islets was significantly stimulated at high glucose concentrations. Moreover, significant incorporation of label from [3H]putrescine was also detected in gamma-aminobutyric acid. This incorporation, however, was not stimulated by high glucose. Possible roles for polyamines associated with the secretory granules in insulin-producing cells are discussed.

AB - Two recently developed fluorescence cytochemical methods, specific for spermidine and spermine, were used to localize polyamines in the endocrine pancreas. The polyamines were restricted to the insulin-producing beta-cells and were mainly associated with the secretory granules. Chemical polyamine determinations carried out on isolated rat and mouse pancreatic islets revealed large amounts of polyamines. Compared with extracts of whole pancreas, the islets contained very high concentrations of spermine relative to spermidine. Biosynthesis of polyamines from [3H]ornithine or from [3H]putrescine in isolated islets was significantly stimulated at high glucose concentrations. Moreover, significant incorporation of label from [3H]putrescine was also detected in gamma-aminobutyric acid. This incorporation, however, was not stimulated by high glucose. Possible roles for polyamines associated with the secretory granules in insulin-producing cells are discussed.

KW - Animals

KW - Cells, Cultured

KW - Glucose

KW - Immunoenzyme Techniques

KW - Insulin

KW - Islets of Langerhans

KW - Mice

KW - Ornithine

KW - Putrescine

KW - Rats

KW - Rats, Inbred Strains

KW - Spermidine

KW - Spermine

M3 - Journal article

C2 - 3541909

VL - 238

SP - 43

EP - 47

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 1

ER -

ID: 47974988