Large, but not small, antigens require time- and temperature-dependent processing in accessory cells before they can be recognized by T cells
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Large, but not small, antigens require time- and temperature-dependent processing in accessory cells before they can be recognized by T cells. / Buus, S; Werdelin, O.
I: APMIS : Acta pathologica, microbiologica et immunologica Scandinavica. Supplementum, Bind 94, Nr. 1, 1986, s. 17-24.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Large, but not small, antigens require time- and temperature-dependent processing in accessory cells before they can be recognized by T cells
AU - Buus, S
AU - Werdelin, O
N1 - Keywords: Angiotensin III; Animals; Antigen-Presenting Cells; Antigens; Chloroquine; Dinitrobenzenes; Formaldehyde; Guinea Pigs; Interleukin-1; Molecular Weight; Ovalbumin; Polylysine; Polymers; T-Lymphocytes; Temperature; Time Factors; Tuberculin
PY - 1986
Y1 - 1986
N2 - We have studied if antigens of different size and structure all require processing in antigen-presenting cells of guinea-pigs before they can be recognized by T cells. The method of mild paraformaldehyde fixation was used to stop antigen-processing in the antigen-presenting cells. As a measure of antigen presentation we used the proliferative response of appropriately primed T cells during a co-culture with the paraformaldehyde-fixed and antigen-exposed presenting cells. We demonstrate that the large synthetic polypeptide antigen, dinitrophenyl-poly-L-lysine, requires processing. After an initial time-lag of 30 min this antigen is fully processed within 2 to 4 of culture at 37 degrees C. In contrast, the immunogenic heptapeptide, angiotensin III, can be presented by pre-fixed accessory cells, viz. without any prior processing. Antigen processing was found to be temperature-dependent and consequently energy-requiring. Processing is strongly inhibited by the lysosomotrophic drug, chloroquine, suggesting a lysosomal involvement in antigen processing. The existence of a minor, non-lysosomal pathway is suggested, since small amounts of antigen were processed even at 10 degrees C, at which temperature no transport to and from the lysosomes can occur.
AB - We have studied if antigens of different size and structure all require processing in antigen-presenting cells of guinea-pigs before they can be recognized by T cells. The method of mild paraformaldehyde fixation was used to stop antigen-processing in the antigen-presenting cells. As a measure of antigen presentation we used the proliferative response of appropriately primed T cells during a co-culture with the paraformaldehyde-fixed and antigen-exposed presenting cells. We demonstrate that the large synthetic polypeptide antigen, dinitrophenyl-poly-L-lysine, requires processing. After an initial time-lag of 30 min this antigen is fully processed within 2 to 4 of culture at 37 degrees C. In contrast, the immunogenic heptapeptide, angiotensin III, can be presented by pre-fixed accessory cells, viz. without any prior processing. Antigen processing was found to be temperature-dependent and consequently energy-requiring. Processing is strongly inhibited by the lysosomotrophic drug, chloroquine, suggesting a lysosomal involvement in antigen processing. The existence of a minor, non-lysosomal pathway is suggested, since small amounts of antigen were processed even at 10 degrees C, at which temperature no transport to and from the lysosomes can occur.
M3 - Journal article
C2 - 3487199
VL - 94
SP - 17
EP - 24
JO - APMIS. Supplementum
JF - APMIS. Supplementum
SN - 0903-465X
IS - 1
ER -
ID: 9948200