Joint Estimates of Heterozygosity and Runs of Homozygosity for Modern and Ancient Samples

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Joint Estimates of Heterozygosity and Runs of Homozygosity for Modern and Ancient Samples. / Renaud, Gabriel; Hanghøj, Kristian; Korneliussen, Thorfinn Sand; Willerslev, Eske; Orlando, Ludovic.

I: Genetics, Bind 212, Nr. 3, 2019, s. 587-614.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Renaud, G, Hanghøj, K, Korneliussen, TS, Willerslev, E & Orlando, L 2019, 'Joint Estimates of Heterozygosity and Runs of Homozygosity for Modern and Ancient Samples', Genetics, bind 212, nr. 3, s. 587-614. https://doi.org/10.1534/genetics.119.302057

APA

Renaud, G., Hanghøj, K., Korneliussen, T. S., Willerslev, E., & Orlando, L. (2019). Joint Estimates of Heterozygosity and Runs of Homozygosity for Modern and Ancient Samples. Genetics, 212(3), 587-614. https://doi.org/10.1534/genetics.119.302057

Vancouver

Renaud G, Hanghøj K, Korneliussen TS, Willerslev E, Orlando L. Joint Estimates of Heterozygosity and Runs of Homozygosity for Modern and Ancient Samples. Genetics. 2019;212(3):587-614. https://doi.org/10.1534/genetics.119.302057

Author

Renaud, Gabriel ; Hanghøj, Kristian ; Korneliussen, Thorfinn Sand ; Willerslev, Eske ; Orlando, Ludovic. / Joint Estimates of Heterozygosity and Runs of Homozygosity for Modern and Ancient Samples. I: Genetics. 2019 ; Bind 212, Nr. 3. s. 587-614.

Bibtex

@article{bd410eef3b63458da2a5dbf1adeedbb5,
title = "Joint Estimates of Heterozygosity and Runs of Homozygosity for Modern and Ancient Samples",
abstract = "Both the total amount and the distribution of heterozygous sites within individual genomes are informative about the genetic diversity of the population they belong to. Detecting true heterozygous sites in ancient genomes is complicated by the generally limited coverage achieved and the presence of post-mortem damage inflating sequencing errors. Additionally, large runs of homozygosity found in the genomes of particularly inbred individuals and of domestic animals can skew estimates of genome-wide heterozygosity rates. Current computational tools aimed at estimating runs of homozygosity and genome-wide heterozygosity levels are generally sensitive to such limitations. Here, we introduce ROHan, a probabilistic method which substantially improves the estimate of heterozygosity rates both genome-wide and for genomic local windows. It combines a local Bayesian model and a Hidden Markov Model at the genome-wide level and can work both on modern and ancient samples. We show that our algorithm outperforms currently available methods for predicting heterozygosity rates for ancient samples. Specifically, ROHan can delineate large runs of homozygosity (at megabase scales) and produce a reliable confidence interval for the genome-wide rate of heterozygosity outside of such regions from modern genomes with a depth of coverage as low as 5-6× and down to 7-8× for ancient samples showing moderate DNA damage. We apply ROHan to a series of modern and ancient genomes previously published and revise available estimates of heterozygosity for humans, chimpanzees and horses.",
keywords = "Faculty of Science, Inbreeding, Heterozygosity, Effective population size, Ancient DNA, Runs of Heterozygosity",
author = "Gabriel Renaud and Kristian Hangh{\o}j and Korneliussen, {Thorfinn Sand} and Eske Willerslev and Ludovic Orlando",
note = "Copyright {\circledC} 2019 by the Genetics Society of America.",
year = "2019",
doi = "10.1534/genetics.119.302057",
language = "English",
volume = "212",
pages = "587--614",
journal = "Genetics",
issn = "1943-2631",
publisher = "The Genetics Society of America (GSA)",
number = "3",

}

RIS

TY - JOUR

T1 - Joint Estimates of Heterozygosity and Runs of Homozygosity for Modern and Ancient Samples

AU - Renaud, Gabriel

AU - Hanghøj, Kristian

AU - Korneliussen, Thorfinn Sand

AU - Willerslev, Eske

AU - Orlando, Ludovic

N1 - Copyright © 2019 by the Genetics Society of America.

PY - 2019

Y1 - 2019

N2 - Both the total amount and the distribution of heterozygous sites within individual genomes are informative about the genetic diversity of the population they belong to. Detecting true heterozygous sites in ancient genomes is complicated by the generally limited coverage achieved and the presence of post-mortem damage inflating sequencing errors. Additionally, large runs of homozygosity found in the genomes of particularly inbred individuals and of domestic animals can skew estimates of genome-wide heterozygosity rates. Current computational tools aimed at estimating runs of homozygosity and genome-wide heterozygosity levels are generally sensitive to such limitations. Here, we introduce ROHan, a probabilistic method which substantially improves the estimate of heterozygosity rates both genome-wide and for genomic local windows. It combines a local Bayesian model and a Hidden Markov Model at the genome-wide level and can work both on modern and ancient samples. We show that our algorithm outperforms currently available methods for predicting heterozygosity rates for ancient samples. Specifically, ROHan can delineate large runs of homozygosity (at megabase scales) and produce a reliable confidence interval for the genome-wide rate of heterozygosity outside of such regions from modern genomes with a depth of coverage as low as 5-6× and down to 7-8× for ancient samples showing moderate DNA damage. We apply ROHan to a series of modern and ancient genomes previously published and revise available estimates of heterozygosity for humans, chimpanzees and horses.

AB - Both the total amount and the distribution of heterozygous sites within individual genomes are informative about the genetic diversity of the population they belong to. Detecting true heterozygous sites in ancient genomes is complicated by the generally limited coverage achieved and the presence of post-mortem damage inflating sequencing errors. Additionally, large runs of homozygosity found in the genomes of particularly inbred individuals and of domestic animals can skew estimates of genome-wide heterozygosity rates. Current computational tools aimed at estimating runs of homozygosity and genome-wide heterozygosity levels are generally sensitive to such limitations. Here, we introduce ROHan, a probabilistic method which substantially improves the estimate of heterozygosity rates both genome-wide and for genomic local windows. It combines a local Bayesian model and a Hidden Markov Model at the genome-wide level and can work both on modern and ancient samples. We show that our algorithm outperforms currently available methods for predicting heterozygosity rates for ancient samples. Specifically, ROHan can delineate large runs of homozygosity (at megabase scales) and produce a reliable confidence interval for the genome-wide rate of heterozygosity outside of such regions from modern genomes with a depth of coverage as low as 5-6× and down to 7-8× for ancient samples showing moderate DNA damage. We apply ROHan to a series of modern and ancient genomes previously published and revise available estimates of heterozygosity for humans, chimpanzees and horses.

KW - Faculty of Science

KW - Inbreeding

KW - Heterozygosity

KW - Effective population size

KW - Ancient DNA

KW - Runs of Heterozygosity

U2 - 10.1534/genetics.119.302057

DO - 10.1534/genetics.119.302057

M3 - Journal article

C2 - 31088861

VL - 212

SP - 587

EP - 614

JO - Genetics

JF - Genetics

SN - 1943-2631

IS - 3

ER -

ID: 224301158