Intrinsic anti-Stokes emission in living HeLa cells

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Standard

Intrinsic anti-Stokes emission in living HeLa cells. / Kacenauskaite, Laura; Gabrielaitis, Dovydas; Bærentsen, Nicolai; Martinez, Karen L.; Vosch, Tom; Laursen, Bo W.

I: PLoS ONE, Bind 15, Nr. 3, e0230441, 01.01.2020.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Kacenauskaite, L, Gabrielaitis, D, Bærentsen, N, Martinez, KL, Vosch, T & Laursen, BW 2020, 'Intrinsic anti-Stokes emission in living HeLa cells', PLoS ONE, bind 15, nr. 3, e0230441. https://doi.org/10.1371/journal.pone.0230441

APA

Kacenauskaite, L., Gabrielaitis, D., Bærentsen, N., Martinez, K. L., Vosch, T., & Laursen, B. W. (2020). Intrinsic anti-Stokes emission in living HeLa cells. PLoS ONE, 15(3), [e0230441]. https://doi.org/10.1371/journal.pone.0230441

Vancouver

Kacenauskaite L, Gabrielaitis D, Bærentsen N, Martinez KL, Vosch T, Laursen BW. Intrinsic anti-Stokes emission in living HeLa cells. PLoS ONE. 2020 jan. 1;15(3). e0230441. https://doi.org/10.1371/journal.pone.0230441

Author

Kacenauskaite, Laura ; Gabrielaitis, Dovydas ; Bærentsen, Nicolai ; Martinez, Karen L. ; Vosch, Tom ; Laursen, Bo W. / Intrinsic anti-Stokes emission in living HeLa cells. I: PLoS ONE. 2020 ; Bind 15, Nr. 3.

Bibtex

@article{f106f32f145f441daadf5466fa828e5d,
title = "Intrinsic anti-Stokes emission in living HeLa cells",
abstract = "Intrinsic fluorescence of biological material, also called auto-fluorescence, is a well-known phenomenon and has in recent years been used for imaging, diagnostics and cell viability studies. Here we show that in addition to commonly observed auto-fluorescence, intrinsic anti-Stokes emission can also be observed under 560 nm or 633 nm excitation. The anti-Stokes emission is shown to be spatially located on/in the mitochondria. The findings presented here show that sensitive imaging experiments e.g. single molecule experiments or two-photon excitation imaging can be compromised if intracellular anti-Stokes emission is not accounted for. On the other hand, we suggest that this anti-Stokes emission could be exploited as an additional modality for mitochondria visualization and cell viability investigation even in systems that are already labeled with commonly used fluorophores that rely on normal Stokes-based detection.",
author = "Laura Kacenauskaite and Dovydas Gabrielaitis and Nicolai B{\ae}rentsen and Martinez, {Karen L.} and Tom Vosch and Laursen, {Bo W.}",
year = "2020",
month = jan,
day = "1",
doi = "10.1371/journal.pone.0230441",
language = "English",
volume = "15",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

RIS

TY - JOUR

T1 - Intrinsic anti-Stokes emission in living HeLa cells

AU - Kacenauskaite, Laura

AU - Gabrielaitis, Dovydas

AU - Bærentsen, Nicolai

AU - Martinez, Karen L.

AU - Vosch, Tom

AU - Laursen, Bo W.

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Intrinsic fluorescence of biological material, also called auto-fluorescence, is a well-known phenomenon and has in recent years been used for imaging, diagnostics and cell viability studies. Here we show that in addition to commonly observed auto-fluorescence, intrinsic anti-Stokes emission can also be observed under 560 nm or 633 nm excitation. The anti-Stokes emission is shown to be spatially located on/in the mitochondria. The findings presented here show that sensitive imaging experiments e.g. single molecule experiments or two-photon excitation imaging can be compromised if intracellular anti-Stokes emission is not accounted for. On the other hand, we suggest that this anti-Stokes emission could be exploited as an additional modality for mitochondria visualization and cell viability investigation even in systems that are already labeled with commonly used fluorophores that rely on normal Stokes-based detection.

AB - Intrinsic fluorescence of biological material, also called auto-fluorescence, is a well-known phenomenon and has in recent years been used for imaging, diagnostics and cell viability studies. Here we show that in addition to commonly observed auto-fluorescence, intrinsic anti-Stokes emission can also be observed under 560 nm or 633 nm excitation. The anti-Stokes emission is shown to be spatially located on/in the mitochondria. The findings presented here show that sensitive imaging experiments e.g. single molecule experiments or two-photon excitation imaging can be compromised if intracellular anti-Stokes emission is not accounted for. On the other hand, we suggest that this anti-Stokes emission could be exploited as an additional modality for mitochondria visualization and cell viability investigation even in systems that are already labeled with commonly used fluorophores that rely on normal Stokes-based detection.

U2 - 10.1371/journal.pone.0230441

DO - 10.1371/journal.pone.0230441

M3 - Journal article

C2 - 32176729

AN - SCOPUS:85081954130

VL - 15

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 3

M1 - e0230441

ER -

ID: 241057879