Interaction of 7SK with the Smn complex modulates snRNP production
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Interaction of 7SK with the Smn complex modulates snRNP production. / Ji, Changhe; Bader, Jakob; Ramanathan, Pradhipa; Hennlein, Luisa; Meissner, Felix; Jablonka, Sibylle; Mann, Matthias; Fischer, Utz; Sendtner, Michael; Briese, Michael.
I: Nature Communications, Bind 12, Nr. 1, 1278, 2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Interaction of 7SK with the Smn complex modulates snRNP production
AU - Ji, Changhe
AU - Bader, Jakob
AU - Ramanathan, Pradhipa
AU - Hennlein, Luisa
AU - Meissner, Felix
AU - Jablonka, Sibylle
AU - Mann, Matthias
AU - Fischer, Utz
AU - Sendtner, Michael
AU - Briese, Michael
PY - 2021
Y1 - 2021
N2 - Gene expression requires tight coordination of the molecular machineries that mediate transcription and splicing. While the interplay between transcription kinetics and spliceosome fidelity has been investigated before, less is known about mechanisms regulating the assembly of the spliceosomal machinery in response to transcription changes. Here, we report an association of the Smn complex, which mediates spliceosomal snRNP biogenesis, with the 7SK complex involved in transcriptional regulation. We found that Smn interacts with the 7SK core components Larp7 and Mepce and specifically associates with 7SK subcomplexes containing hnRNP R. The association between Smn and 7SK complexes is enhanced upon transcriptional inhibition leading to reduced production of snRNPs. Taken together, our findings reveal a functional association of Smn and 7SK complexes that is governed by global changes in transcription. Thus, in addition to its canonical nuclear role in transcriptional regulation, 7SK has cytosolic functions in fine-tuning spliceosome production according to transcriptional demand.
AB - Gene expression requires tight coordination of the molecular machineries that mediate transcription and splicing. While the interplay between transcription kinetics and spliceosome fidelity has been investigated before, less is known about mechanisms regulating the assembly of the spliceosomal machinery in response to transcription changes. Here, we report an association of the Smn complex, which mediates spliceosomal snRNP biogenesis, with the 7SK complex involved in transcriptional regulation. We found that Smn interacts with the 7SK core components Larp7 and Mepce and specifically associates with 7SK subcomplexes containing hnRNP R. The association between Smn and 7SK complexes is enhanced upon transcriptional inhibition leading to reduced production of snRNPs. Taken together, our findings reveal a functional association of Smn and 7SK complexes that is governed by global changes in transcription. Thus, in addition to its canonical nuclear role in transcriptional regulation, 7SK has cytosolic functions in fine-tuning spliceosome production according to transcriptional demand.
KW - Animals
KW - Cells, Cultured
KW - HEK293 Cells
KW - HeLa Cells
KW - Humans
KW - Mice
KW - Motor Neurons/metabolism
KW - RNA, Long Noncoding/genetics
KW - Ribonucleoproteins, Small Nuclear/genetics
KW - SMN Complex Proteins/genetics
KW - Tandem Mass Spectrometry
KW - Transcription, Genetic/genetics
U2 - 10.1038/s41467-021-21529-1
DO - 10.1038/s41467-021-21529-1
M3 - Journal article
C2 - 33627647
VL - 12
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 1278
ER -
ID: 259827810