TY - JOUR

T1 - Insights into high hydrostatic pressure pre-treatment generating a more efficient catalytic mode of maltogenic α-amylase

T2 - Effect of multi-level structure on retrogradation properties of maize starch

AU - Liu, Zhenyu

AU - Zhong, Yuyue

AU - Khakimov, Bekzod

AU - Fu, Yongxia

AU - Czaja, Tomasz Pawel

AU - Kirkensgaard, Jacob Judas Kain

AU - Blennow, Andreas

AU - Shen, Qun

AU - Engelsen, Søren Balling

PY - 2023

Y1 - 2023

N2 - Maltogenic alpha-amylase (MA) is an enzyme used to retard starch retrogradation in the baking industry. However, sometimes it exhibits low efficiency which may restrict the enzymatic application. This study investigates the effects of using High hydrostatic pressure (HHP) as a pre-treatment to promote MA catalysis. The results showed that HHP resulted in more indentations and fissures of the starch granules promoting the susceptibility of MA. While MA treatment alone introduced short malto-oligosaccharide chains with degree of polymerization (DP) 1-5, the combined HHP-MA treatment exhibited a higher proportion of DP 1-9, but a lower proportion of DP 13-24. Furthermore, when increasing the pressure, the ratio of alpha-1,6 to alpha-1,4 glycosidic bonds increased in the MA-treated starch. MA modification on the HHP-pretreated starch showed increasing gelatinization temperature and enthalpy, while it had a reverse impact on long-term retrogradation enthalpy due to the shortening of the outer chains of amylopectin. LF-NMR was applied to analyze the water mobility and compartmentalization in starch gel systems after 1-and 7-days storage. Compared with single MA-treated system, HHP-MA treated starches showed no significant changes in the value of the transverse relaxation times, but less amount of bulk water was detected due to higher amounts of soluble small oligosaccharides. In conclusion, HHP pre-treatment combined with MA could improve the water holding properties of retrograded starch gels and that HHP prior to MA catalysis provides an efficient alternative method to modify starch-based food products with anti-stalling properties.

AB - Maltogenic alpha-amylase (MA) is an enzyme used to retard starch retrogradation in the baking industry. However, sometimes it exhibits low efficiency which may restrict the enzymatic application. This study investigates the effects of using High hydrostatic pressure (HHP) as a pre-treatment to promote MA catalysis. The results showed that HHP resulted in more indentations and fissures of the starch granules promoting the susceptibility of MA. While MA treatment alone introduced short malto-oligosaccharide chains with degree of polymerization (DP) 1-5, the combined HHP-MA treatment exhibited a higher proportion of DP 1-9, but a lower proportion of DP 13-24. Furthermore, when increasing the pressure, the ratio of alpha-1,6 to alpha-1,4 glycosidic bonds increased in the MA-treated starch. MA modification on the HHP-pretreated starch showed increasing gelatinization temperature and enthalpy, while it had a reverse impact on long-term retrogradation enthalpy due to the shortening of the outer chains of amylopectin. LF-NMR was applied to analyze the water mobility and compartmentalization in starch gel systems after 1-and 7-days storage. Compared with single MA-treated system, HHP-MA treated starches showed no significant changes in the value of the transverse relaxation times, but less amount of bulk water was detected due to higher amounts of soluble small oligosaccharides. In conclusion, HHP pre-treatment combined with MA could improve the water holding properties of retrograded starch gels and that HHP prior to MA catalysis provides an efficient alternative method to modify starch-based food products with anti-stalling properties.

KW - Maltogenic ?-amylase

KW - High hydrostatic pressure

KW - Less-crystalline granular starch

KW - Anti-aging

KW - Water mobility

KW - PHYSICOCHEMICAL PROPERTIES

KW - RICE STARCH

KW - CRYSTALLINE

KW - IMPACT

U2 - 10.1016/j.foodhyd.2023.108480

DO - 10.1016/j.foodhyd.2023.108480

M3 - Journal article

VL - 138

JO - Food Hydrocolloids

JF - Food Hydrocolloids

SN - 0268-005X

M1 - 108480

ER -