In-Depth Proteomic Map of Innate Lymphoid Cells from Healthy Human Skin and Blood

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Marcel B.M. Teunissen
Line B. Pilgaard Møller
Løvendorf, Marianne Bengtson
Skov, Lone
Bonefeld, Charlotte Menne
Marcel W. Bekkenk
Rachael A. Clark
Mann, Matthias
Dyring-Andersen, Beatrice

Innate lymphoid cells (ILCs) are essential players in the skin-associated immune system, nevertheless little is known about their proteomes and proteomic diversity. In this study, we describe about 6,600 proteins constitutively expressed by ILC2s and ILC3s from healthy human skin and blood using state-of-the-art proteomics. Although the vast majority of proteins was expressed by both ILC subsets and in both compartments, the skin ILC2s and ILC3s were more distinct than their counterparts in blood. Only skin ILC3s expressed uniquely detected proteins. Our in-depth proteomic dataset allowed us to define the cluster of differentiation marker profiles of the ILC subsets, explore distribution and abundance of proteins known to have immunological functions, as well as identify subset-specific proteins that have not previously been implicated in ILC biology. Taken together, our analyses substantially expand understanding of the protein expression signatures of ILC subsets. Going forward, these proteomic datasets will serve as valuable resources for future studies of ILC biology.

Originalsprog	Engelsk
Tidsskrift	Journal of Investigative Dermatology
Vol/bind	144
Udgave nummer	2
Antal sider	18
ISSN	0022-202X
DOI	https://doi.org/10.1016/j.jid.2023.07.011
Status	Udgivet - 2024

Bibliografisk note

Funding Information:
The authors thank Jeppe Madsen at the NNF CPR Mass Spectrometry Platform, University of Copenhagen for the skilled technical assistance. The authors thank Mihai Mannolache and Martin Dyring-Andersen for the development of a searchable interface on http://skin.science . The authors thank Juliet Percival for helping with illustrations in Figure 1 a. This work was supported by Novo Nordisk Foundation (grants NNF14CC0001 and NNF15CC0001 to BD-A), Lundbeck Foundation (grant R182-2014-3641 to BD-A), NIH/NIAMS (grant P30AR069625 to RAC), and NIH/NIAID (grant R01AI127654 to RAC). BD-A is supported by the Aage Bangs Foundation and the A.P. Møller Foundation for the Advancement of Medical Sciences, MM is supported by the Max-Planck Society for the Advancement of Science and BD-A, MBL, and LS are supported by the Leo Foundation. The guarantor responsible for addressing any concerns that arise after publication of this study (MBMT).

Funding Information:
The authors thank Jeppe Madsen at the NNF CPR Mass Spectrometry Platform, University of Copenhagen for the skilled technical assistance. The authors thank Mihai Mannolache and Martin Dyring-Andersen for the development of a searchable interface on http://skin.science. The authors thank Juliet Percival for helping with illustrations in Figure 1a. This work was supported by Novo Nordisk Foundation (grants NNF14CC0001 and NNF15CC0001 to BD-A), Lundbeck Foundation (grant R182-2014-3641 to BD-A), NIH/NIAMS (grant P30AR069625 to RAC), and NIH/NIAID (grant R01AI127654 to RAC). BD-A is supported by the Aage Bangs Foundation and the A.P. Møller Foundation for the Advancement of Medical Sciences, MM is supported by the Max-Planck Society for the Advancement of Science and BD-A, MBL, and LS are supported by the Leo Foundation. The guarantor responsible for addressing any concerns that arise after publication of this study (MBMT). Conceptualization: MBMT, BD-A; Formal Analysis: MBMT, LBPM, MBL, BD-A; Investigation: MBMT, LBPM, MBL, BD-A; Methodology: MBMT, LBPM; Visualization: MBMT, LBPM, MBL, BD-A; Writing - Original Draft Preparation: MBMT, LBPM, BD-A; Writing - Review and Editing: MBMT, LBPM, MBL, LS, CMB, MWB, RAC, MM, BD-A

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© 2023 The Authors

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