Increased RNA polymerase availability directs resources towards growth at the expense of maintenance
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Increased RNA polymerase availability directs resources towards growth at the expense of maintenance. / Gummesson, Bertil; Magnusson, Lisa U.; Lovmar, Martin; Kvint, Kristian; Persson, Örjan; Ballesteros, Manuel; Farewell, Anne; Nyström, Thomas.
I: EMBO Journal, Bind 28, Nr. 15, 01.08.2009, s. 2209-2219.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Increased RNA polymerase availability directs resources towards growth at the expense of maintenance
AU - Gummesson, Bertil
AU - Magnusson, Lisa U.
AU - Lovmar, Martin
AU - Kvint, Kristian
AU - Persson, Örjan
AU - Ballesteros, Manuel
AU - Farewell, Anne
AU - Nyström, Thomas
PY - 2009/8/1
Y1 - 2009/8/1
N2 - Nutritionally induced changes in RNA polymerase availability have been hypothesized to be an evolutionary primeval mechanism for regulation of gene expression and several contrasting models have been proposed to explain how such 'passive' regulation might occur. We demonstrate here that ectopically elevating Escherichia coli RNA polymerase (Eσ70) levels causes an increased expression and promoter occupancy of ribosomal genes at the expense of stress-defense genes and amino acid biosynthetic operons. Phenotypically, cells overproducing Eσ70 favours growth and reproduction at the expense of motility and damage protection; a response reminiscent of cells with no or diminished levels of the alarmone guanosine tetraphosphate (ppGpp). Consistently, we show that cells lacking ppGpp displayed markedly elevated levels of free Eσ70 compared with wild-type cells and that the repression of ribosomal RNA expression and reduced growth rate of mutants with constitutively elevated levels of ppGpp can be suppressed by overproducing Eσ70. We conclude that ppGpp modulates the levels of free Eσ70 and that this is an integral part of the alarmone's means of regulating a trade-off between growth and maintenance.
AB - Nutritionally induced changes in RNA polymerase availability have been hypothesized to be an evolutionary primeval mechanism for regulation of gene expression and several contrasting models have been proposed to explain how such 'passive' regulation might occur. We demonstrate here that ectopically elevating Escherichia coli RNA polymerase (Eσ70) levels causes an increased expression and promoter occupancy of ribosomal genes at the expense of stress-defense genes and amino acid biosynthetic operons. Phenotypically, cells overproducing Eσ70 favours growth and reproduction at the expense of motility and damage protection; a response reminiscent of cells with no or diminished levels of the alarmone guanosine tetraphosphate (ppGpp). Consistently, we show that cells lacking ppGpp displayed markedly elevated levels of free Eσ70 compared with wild-type cells and that the repression of ribosomal RNA expression and reduced growth rate of mutants with constitutively elevated levels of ppGpp can be suppressed by overproducing Eσ70. We conclude that ppGpp modulates the levels of free Eσ70 and that this is an integral part of the alarmone's means of regulating a trade-off between growth and maintenance.
KW - Passive regulation
KW - ppGpp
KW - RNA polymerase
KW - Stringent control
KW - Transcriptional trade-off
UR - http://www.scopus.com/inward/record.url?scp=68249138705&partnerID=8YFLogxK
U2 - 10.1038/emboj.2009.181
DO - 10.1038/emboj.2009.181
M3 - Journal article
C2 - 19574956
AN - SCOPUS:68249138705
VL - 28
SP - 2209
EP - 2219
JO - E M B O Journal
JF - E M B O Journal
SN - 0261-4189
IS - 15
ER -
ID: 203861885