Identification of ige-binding egg white proteins: Comparison of results obtained by different methods

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Standard

Identification of ige-binding egg white proteins : Comparison of results obtained by different methods. / Aabin, B.; Poulsen, L. K.; Ebbehøj, K.; Nørgaard, A.; Frøkiœr, H.; Bindslev-Jensen, C.; Barkholt, V.

I: International Archives of Allergy and Immunology, Bind 109, Nr. 1, 1996, s. 50-57.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Aabin, B, Poulsen, LK, Ebbehøj, K, Nørgaard, A, Frøkiœr, H, Bindslev-Jensen, C & Barkholt, V 1996, 'Identification of ige-binding egg white proteins: Comparison of results obtained by different methods', International Archives of Allergy and Immunology, bind 109, nr. 1, s. 50-57. https://doi.org/10.1159/000237231

APA

Aabin, B., Poulsen, L. K., Ebbehøj, K., Nørgaard, A., Frøkiœr, H., Bindslev-Jensen, C., & Barkholt, V. (1996). Identification of ige-binding egg white proteins: Comparison of results obtained by different methods. International Archives of Allergy and Immunology, 109(1), 50-57. https://doi.org/10.1159/000237231

Vancouver

Aabin B, Poulsen LK, Ebbehøj K, Nørgaard A, Frøkiœr H, Bindslev-Jensen C o.a. Identification of ige-binding egg white proteins: Comparison of results obtained by different methods. International Archives of Allergy and Immunology. 1996;109(1):50-57. https://doi.org/10.1159/000237231

Author

Aabin, B. ; Poulsen, L. K. ; Ebbehøj, K. ; Nørgaard, A. ; Frøkiœr, H. ; Bindslev-Jensen, C. ; Barkholt, V. / Identification of ige-binding egg white proteins : Comparison of results obtained by different methods. I: International Archives of Allergy and Immunology. 1996 ; Bind 109, Nr. 1. s. 50-57.

Bibtex

@article{1d3e2a7fccf742ee8c3491aebb91d151,
title = "Identification of ige-binding egg white proteins: Comparison of results obtained by different methods",
abstract = "The binding of IgE to egg white proteins was investigated for 34 sera from adults with a positive case history and/or positive RAST towards egg, and the impact of experimental conditions on IgE binding in commonly used methods was studied. Radioimmunoblotting after SDS-PAGE of both reduced and unreduced egg white extracts showed complex reaction patterns. The results were confirmed by crossed radioimmunoelectrophoresis (CRIE). Radio dot immunobinding was used to investigate the effect of treatment of allergens for SDS-PAGE and to evaluate the other methods. As a conclusion, the use of combinations of at least two methods is recommended for the identification of IgE-binding egg white proteins. Of the 34 sera, 18 reacted with ovotransferrin, 13 with ovomucoid, 11 with ovalbumin and 5 with lysozyme. The amounts of IgE bound to ovalbumin and lysozyme were generally lower than the amounts bound to ovotransferrin and ovomucoid.",
keywords = "Crossed radioimmunoelectrophoresis, Dot immunobinding, Egg white, Food allergens, IgE, SDS-PAGE",
author = "B. Aabin and Poulsen, {L. K.} and K. Ebbeh{\o}j and A. N{\o}rgaard and H. Fr{\o}ki{\oe}r and C. Bindslev-Jensen and V. Barkholt",
year = "1996",
doi = "10.1159/000237231",
language = "English",
volume = "109",
pages = "50--57",
journal = "International Archives of Allergy and Immunology",
issn = "1018-2438",
publisher = "S Karger AG",
number = "1",

}

RIS

TY - JOUR

T1 - Identification of ige-binding egg white proteins

T2 - Comparison of results obtained by different methods

AU - Aabin, B.

AU - Poulsen, L. K.

AU - Ebbehøj, K.

AU - Nørgaard, A.

AU - Frøkiœr, H.

AU - Bindslev-Jensen, C.

AU - Barkholt, V.

PY - 1996

Y1 - 1996

N2 - The binding of IgE to egg white proteins was investigated for 34 sera from adults with a positive case history and/or positive RAST towards egg, and the impact of experimental conditions on IgE binding in commonly used methods was studied. Radioimmunoblotting after SDS-PAGE of both reduced and unreduced egg white extracts showed complex reaction patterns. The results were confirmed by crossed radioimmunoelectrophoresis (CRIE). Radio dot immunobinding was used to investigate the effect of treatment of allergens for SDS-PAGE and to evaluate the other methods. As a conclusion, the use of combinations of at least two methods is recommended for the identification of IgE-binding egg white proteins. Of the 34 sera, 18 reacted with ovotransferrin, 13 with ovomucoid, 11 with ovalbumin and 5 with lysozyme. The amounts of IgE bound to ovalbumin and lysozyme were generally lower than the amounts bound to ovotransferrin and ovomucoid.

AB - The binding of IgE to egg white proteins was investigated for 34 sera from adults with a positive case history and/or positive RAST towards egg, and the impact of experimental conditions on IgE binding in commonly used methods was studied. Radioimmunoblotting after SDS-PAGE of both reduced and unreduced egg white extracts showed complex reaction patterns. The results were confirmed by crossed radioimmunoelectrophoresis (CRIE). Radio dot immunobinding was used to investigate the effect of treatment of allergens for SDS-PAGE and to evaluate the other methods. As a conclusion, the use of combinations of at least two methods is recommended for the identification of IgE-binding egg white proteins. Of the 34 sera, 18 reacted with ovotransferrin, 13 with ovomucoid, 11 with ovalbumin and 5 with lysozyme. The amounts of IgE bound to ovalbumin and lysozyme were generally lower than the amounts bound to ovotransferrin and ovomucoid.

KW - Crossed radioimmunoelectrophoresis

KW - Dot immunobinding

KW - Egg white

KW - Food allergens

KW - IgE

KW - SDS-PAGE

U2 - 10.1159/000237231

DO - 10.1159/000237231

M3 - Journal article

C2 - 8527951

AN - SCOPUS:0029655315

VL - 109

SP - 50

EP - 57

JO - International Archives of Allergy and Immunology

JF - International Archives of Allergy and Immunology

SN - 1018-2438

IS - 1

ER -

ID: 328565993