Identification of a growth hormone-responsive STAT5-binding element in the rat insulin 1 gene

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Standard

Identification of a growth hormone-responsive STAT5-binding element in the rat insulin 1 gene. / Galsgaard, E D; Gouilleux, F; Groner, B; Serup, P; Billestrup, N; Nielsen, Jens Høiriis.

I: Molecular endocrinology (Baltimore, Md.), Bind 10, Nr. 6, 06.1996, s. 652-60.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Galsgaard, ED, Gouilleux, F, Groner, B, Serup, P, Billestrup, N & Nielsen, JH 1996, 'Identification of a growth hormone-responsive STAT5-binding element in the rat insulin 1 gene', Molecular endocrinology (Baltimore, Md.), bind 10, nr. 6, s. 652-60.

APA

Galsgaard, E. D., Gouilleux, F., Groner, B., Serup, P., Billestrup, N., & Nielsen, J. H. (1996). Identification of a growth hormone-responsive STAT5-binding element in the rat insulin 1 gene. Molecular endocrinology (Baltimore, Md.), 10(6), 652-60.

Vancouver

Galsgaard ED, Gouilleux F, Groner B, Serup P, Billestrup N, Nielsen JH. Identification of a growth hormone-responsive STAT5-binding element in the rat insulin 1 gene. Molecular endocrinology (Baltimore, Md.). 1996 jun;10(6):652-60.

Author

Galsgaard, E D ; Gouilleux, F ; Groner, B ; Serup, P ; Billestrup, N ; Nielsen, Jens Høiriis. / Identification of a growth hormone-responsive STAT5-binding element in the rat insulin 1 gene. I: Molecular endocrinology (Baltimore, Md.). 1996 ; Bind 10, Nr. 6. s. 652-60.

Bibtex

@article{fefdde8db94a450d9593841dcf5cab75,
title = "Identification of a growth hormone-responsive STAT5-binding element in the rat insulin 1 gene",
abstract = "GH and PRL stimulate both proliferation and insulin production in pancreatic beta-cells as well as in the rat insulinoma cell line RIN-5AH, We report here that human GH increases insulin mRNA levels in RIN-5AH cells via both somatogenic and lactogenic receptors. GH stimulated the rat insulin 1 promoter activity 2-fold, and this stimulation was abolished by introduction of a block mutation in a gamma-interferon-activated sequence (GAS)-like element (GLE) with the sequence 5'-TTCTGGGAA-3' located in the rat insulin 1 enhancer at position -330 to -322. This element, termed Ins-GLE, was able to confer GH responsiveness to a heterologous promoter. GH induced the binding of two protein complexes to the Ins-GLE. An antibody directed against the transcription factor STAT5 (signal transducer and activator of transcription) supershifted the GH-induced complexes. Furthermore, in COS7 cells transiently transfected with STAT5 and GH receptor cDNAs, it was found that expression of STAT5 was necessary for GH induction of these two DNA-binding complexes. These results suggest that GH stimulates insulin 1 promoter activity by inducing the binding of STAT5 to Ins-GLE.",
keywords = "Animals, Binding Sites, DNA-Binding Proteins, Gene Expression Regulation, Neoplastic, Growth Hormone, Insulin, Insulinoma, Milk Proteins, Promoter Regions, Genetic, Proteins, RNA, Messenger, Rats, STAT5 Transcription Factor, Trans-Activators, Tumor Cells, Cultured, Up-Regulation",
author = "Galsgaard, {E D} and F Gouilleux and B Groner and P Serup and N Billestrup and Nielsen, {Jens H{\o}iriis}",
year = "1996",
month = "6",
language = "English",
volume = "10",
pages = "652--60",
journal = "Molecular Endocrinology",
issn = "0888-8809",
publisher = "Oxford University Press",
number = "6",

}

RIS

TY - JOUR

T1 - Identification of a growth hormone-responsive STAT5-binding element in the rat insulin 1 gene

AU - Galsgaard, E D

AU - Gouilleux, F

AU - Groner, B

AU - Serup, P

AU - Billestrup, N

AU - Nielsen, Jens Høiriis

PY - 1996/6

Y1 - 1996/6

N2 - GH and PRL stimulate both proliferation and insulin production in pancreatic beta-cells as well as in the rat insulinoma cell line RIN-5AH, We report here that human GH increases insulin mRNA levels in RIN-5AH cells via both somatogenic and lactogenic receptors. GH stimulated the rat insulin 1 promoter activity 2-fold, and this stimulation was abolished by introduction of a block mutation in a gamma-interferon-activated sequence (GAS)-like element (GLE) with the sequence 5'-TTCTGGGAA-3' located in the rat insulin 1 enhancer at position -330 to -322. This element, termed Ins-GLE, was able to confer GH responsiveness to a heterologous promoter. GH induced the binding of two protein complexes to the Ins-GLE. An antibody directed against the transcription factor STAT5 (signal transducer and activator of transcription) supershifted the GH-induced complexes. Furthermore, in COS7 cells transiently transfected with STAT5 and GH receptor cDNAs, it was found that expression of STAT5 was necessary for GH induction of these two DNA-binding complexes. These results suggest that GH stimulates insulin 1 promoter activity by inducing the binding of STAT5 to Ins-GLE.

AB - GH and PRL stimulate both proliferation and insulin production in pancreatic beta-cells as well as in the rat insulinoma cell line RIN-5AH, We report here that human GH increases insulin mRNA levels in RIN-5AH cells via both somatogenic and lactogenic receptors. GH stimulated the rat insulin 1 promoter activity 2-fold, and this stimulation was abolished by introduction of a block mutation in a gamma-interferon-activated sequence (GAS)-like element (GLE) with the sequence 5'-TTCTGGGAA-3' located in the rat insulin 1 enhancer at position -330 to -322. This element, termed Ins-GLE, was able to confer GH responsiveness to a heterologous promoter. GH induced the binding of two protein complexes to the Ins-GLE. An antibody directed against the transcription factor STAT5 (signal transducer and activator of transcription) supershifted the GH-induced complexes. Furthermore, in COS7 cells transiently transfected with STAT5 and GH receptor cDNAs, it was found that expression of STAT5 was necessary for GH induction of these two DNA-binding complexes. These results suggest that GH stimulates insulin 1 promoter activity by inducing the binding of STAT5 to Ins-GLE.

KW - Animals

KW - Binding Sites

KW - DNA-Binding Proteins

KW - Gene Expression Regulation, Neoplastic

KW - Growth Hormone

KW - Insulin

KW - Insulinoma

KW - Milk Proteins

KW - Promoter Regions, Genetic

KW - Proteins

KW - RNA, Messenger

KW - Rats

KW - STAT5 Transcription Factor

KW - Trans-Activators

KW - Tumor Cells, Cultured

KW - Up-Regulation

M3 - Journal article

C2 - 8776725

VL - 10

SP - 652

EP - 660

JO - Molecular Endocrinology

JF - Molecular Endocrinology

SN - 0888-8809

IS - 6

ER -

ID: 47972994