Hydrogen bonding versus stacking stabilization by modified nucleobases incorporated in PNA. DNA duplexes

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Hydrogen bonding versus stacking stabilization by modified nucleobases incorporated in PNA. DNA duplexes. / Sen, Anjana; Nielsen, Peter E.

I: Biophysical Chemistry, Bind 141, Nr. 1, 2009, s. 29-33.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Sen, A & Nielsen, PE 2009, 'Hydrogen bonding versus stacking stabilization by modified nucleobases incorporated in PNA. DNA duplexes', Biophysical Chemistry, bind 141, nr. 1, s. 29-33. https://doi.org/10.1016/j.bpc.2008.12.006

APA

Sen, A., & Nielsen, P. E. (2009). Hydrogen bonding versus stacking stabilization by modified nucleobases incorporated in PNA. DNA duplexes. Biophysical Chemistry, 141(1), 29-33. https://doi.org/10.1016/j.bpc.2008.12.006

Vancouver

Sen A, Nielsen PE. Hydrogen bonding versus stacking stabilization by modified nucleobases incorporated in PNA. DNA duplexes. Biophysical Chemistry. 2009;141(1):29-33. https://doi.org/10.1016/j.bpc.2008.12.006

Author

Sen, Anjana ; Nielsen, Peter E. / Hydrogen bonding versus stacking stabilization by modified nucleobases incorporated in PNA. DNA duplexes. I: Biophysical Chemistry. 2009 ; Bind 141, Nr. 1. s. 29-33.

Bibtex

@article{48b95ff05c9e11dea8de000ea68e967b,
title = "Hydrogen bonding versus stacking stabilization by modified nucleobases incorporated in PNA. DNA duplexes",
abstract = "The effects of incorporation of the modified nucleobases, 2,6-diaminopurine (D) (substituting for adenine) and 7-chloro-1,8-naphthyridin-2-(1H)-one (bicyclic thymine, bT) (substituting for thymine), that stabilize PNA.DNA duplex formation by increasing hydrogen bonding and/or base pair stacking interactions have been studied by thermal denaturation in terms of thermodynamics. Although the stabilizing effect of the bT base (in contrast to that of D base) is abolished upon addition of dimethyl formamide, thereby indicating that the stabilization is predominantly due to hydrophobic stacking forces, duplex stabilization was found to be enthalpic for both nucleobases. Increased stabilization (although not fully linearly) was observed with increasing numbers of modified bases, and single base sequence discrimination was only slightly compromised, but showed significant dependence on the sequence context.",
author = "Anjana Sen and Nielsen, {Peter E}",
note = "Keywords: Base Pairing; Base Sequence; DNA; Dimethylformamide; Hydrogen Bonding; Nucleic Acid Denaturation; Peptide Nucleic Acids; Thermodynamics; Thymine; Transition Temperature",
year = "2009",
doi = "10.1016/j.bpc.2008.12.006",
language = "English",
volume = "141",
pages = "29--33",
journal = "Biophysical Chemistry",
issn = "0301-4622",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Hydrogen bonding versus stacking stabilization by modified nucleobases incorporated in PNA. DNA duplexes

AU - Sen, Anjana

AU - Nielsen, Peter E

N1 - Keywords: Base Pairing; Base Sequence; DNA; Dimethylformamide; Hydrogen Bonding; Nucleic Acid Denaturation; Peptide Nucleic Acids; Thermodynamics; Thymine; Transition Temperature

PY - 2009

Y1 - 2009

N2 - The effects of incorporation of the modified nucleobases, 2,6-diaminopurine (D) (substituting for adenine) and 7-chloro-1,8-naphthyridin-2-(1H)-one (bicyclic thymine, bT) (substituting for thymine), that stabilize PNA.DNA duplex formation by increasing hydrogen bonding and/or base pair stacking interactions have been studied by thermal denaturation in terms of thermodynamics. Although the stabilizing effect of the bT base (in contrast to that of D base) is abolished upon addition of dimethyl formamide, thereby indicating that the stabilization is predominantly due to hydrophobic stacking forces, duplex stabilization was found to be enthalpic for both nucleobases. Increased stabilization (although not fully linearly) was observed with increasing numbers of modified bases, and single base sequence discrimination was only slightly compromised, but showed significant dependence on the sequence context.

AB - The effects of incorporation of the modified nucleobases, 2,6-diaminopurine (D) (substituting for adenine) and 7-chloro-1,8-naphthyridin-2-(1H)-one (bicyclic thymine, bT) (substituting for thymine), that stabilize PNA.DNA duplex formation by increasing hydrogen bonding and/or base pair stacking interactions have been studied by thermal denaturation in terms of thermodynamics. Although the stabilizing effect of the bT base (in contrast to that of D base) is abolished upon addition of dimethyl formamide, thereby indicating that the stabilization is predominantly due to hydrophobic stacking forces, duplex stabilization was found to be enthalpic for both nucleobases. Increased stabilization (although not fully linearly) was observed with increasing numbers of modified bases, and single base sequence discrimination was only slightly compromised, but showed significant dependence on the sequence context.

U2 - 10.1016/j.bpc.2008.12.006

DO - 10.1016/j.bpc.2008.12.006

M3 - Journal article

C2 - 19162391

VL - 141

SP - 29

EP - 33

JO - Biophysical Chemistry

JF - Biophysical Chemistry

SN - 0301-4622

IS - 1

ER -

ID: 12703780