Housing-temperature reveals energy intake counter-balances energy expenditure in normal-weight, but not diet-induced obese, male mice

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Most metabolic studies on mice are performed at room temperature, although under these conditions mice, unlike humans, spend considerable energy to maintain core temperature. Here, we characterize the impact of housing temperature on energy expenditure (EE), energy homeostasis and plasma concentrations of appetite- and glucoregulatory hormones in normal-weight and diet-induced obese (DIO) C57BL/6J mice fed chow or 45% high-fat-diet, respectively. Mice were housed for 33 days at 22, 25, 27.5, and 30 °C in an indirect-calorimetry-system. We show that energy expenditure increases linearly from 30 °C towards 22 °C and is ~30% higher at 22 °C in both mouse models. In normal-weight mice, food intake counter-balances EE. In contrast, DIO mice do not reduce food intake when EE is lowered. By end of study, mice at 30 °C, therefore, had higher body weight, fat mass and plasma glycerol and triglycerides than mice at 22 °C. Dysregulated counterbalancing in DIO mice may result from increased pleasure-based eating.

TidsskriftCommunications Biology
Udgave nummer1
Antal sider13
StatusUdgivet - 2022

Bibliografisk note

Funding Information:
We would like to express our gratitude to animal technicians Helle Andersen and Maja Gross Christensen for technical assistance (Global Obesity and Liver Disease, Global Research, Novo Nordisk A/S, Måløv, Denmark), as well as the animal technicians in Novo Nordisk Animal unit (Global Discovery and Development, Novo Nordisk A/S, Måløv, Denmark) for general care-taking. Furthermore, we would like to express our gratitude to Susanne Jørgensen and Johannes Josef Fels (Research Bioanalysis, Global Research Technologies, Novo Nordisk A/S, Måløv, Denmark) for quantification of plasma hormone concentrations.

Publisher Copyright:
© 2022, The Author(s).

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