High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging

Publikation: Bidrag til tidsskrift › Letter › Forskning › fagfællebedømt

Standard

High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging. / Amador, Cristina Isabel; Stannius, Rune Overlund; Røder, Henriette Lyng; Burmølle, Mette.

I: Journal of Microbiological Methods, Bind 190, 106343, 2021.

Publikation: Bidrag til tidsskrift › Letter › Forskning › fagfællebedømt

Harvard

Amador, CI, Stannius, RO, Røder, HL & Burmølle, M 2021, 'High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging', Journal of Microbiological Methods, bind 190, 106343. https://doi.org/10.1016/j.mimet.2021.106343

APA

Amador, C. I., Stannius, R. O., Røder, H. L., & Burmølle, M. (2021). High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging. Journal of Microbiological Methods, 190, [106343]. https://doi.org/10.1016/j.mimet.2021.106343

Vancouver

Amador CI, Stannius RO, Røder HL, Burmølle M. High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging. Journal of Microbiological Methods. 2021;190. 106343. https://doi.org/10.1016/j.mimet.2021.106343

Author

Amador, Cristina Isabel ; Stannius, Rune Overlund ; Røder, Henriette Lyng ; Burmølle, Mette. / High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging. I: Journal of Microbiological Methods. 2021 ; Bind 190.

Bibtex

@article{cc5cdef7e0fa4bb8845f00cc32cc42e5,

title = "High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging",

abstract = "The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.",

author = "Amador, {Cristina Isabel} and Stannius, {Rune Overlund} and R{\o}der, {Henriette Lyng} and Mette Burm{\o}lle",

note = "Copyright {\textcopyright} 2021. Published by Elsevier B.V.",

year = "2021",

doi = "10.1016/j.mimet.2021.106343",

language = "English",

volume = "190",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging

AU - Amador, Cristina Isabel

AU - Stannius, Rune Overlund

AU - Røder, Henriette Lyng

AU - Burmølle, Mette

PY - 2021

Y1 - 2021

N2 - The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.

AB - The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.

U2 - 10.1016/j.mimet.2021.106343

DO - 10.1016/j.mimet.2021.106343

M3 - Letter

C2 - 34619138

VL - 190

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

M1 - 106343

ER -

ID: 281761066