High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires: intra-serovar divergence, inter-serovar convergence, and evidence of attenuation in Leptospira reference collections

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Standard

High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires : intra-serovar divergence, inter-serovar convergence, and evidence of attenuation in Leptospira reference collections. / Tulsiani, Suhella; Craig, S B; Graham, G C; Cobbold, R C; Dohnt, M F; Burns, M-A; Jansen, C C; Leung, L K-P; Field, H E; Smythe, L D.

I: Pathogens and Global Health, Bind 104, Nr. 5, 07.2010, s. 427-37.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Tulsiani, S, Craig, SB, Graham, GC, Cobbold, RC, Dohnt, MF, Burns, M-A, Jansen, CC, Leung, LK-P, Field, HE & Smythe, LD 2010, 'High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires: intra-serovar divergence, inter-serovar convergence, and evidence of attenuation in Leptospira reference collections', Pathogens and Global Health, bind 104, nr. 5, s. 427-37. https://doi.org/10.1179/136485910X12786389891047

APA

Tulsiani, S., Craig, S. B., Graham, G. C., Cobbold, R. C., Dohnt, M. F., Burns, M-A., Jansen, C. C., Leung, L. K-P., Field, H. E., & Smythe, L. D. (2010). High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires: intra-serovar divergence, inter-serovar convergence, and evidence of attenuation in Leptospira reference collections. Pathogens and Global Health, 104(5), 427-37. https://doi.org/10.1179/136485910X12786389891047

Vancouver

Tulsiani S, Craig SB, Graham GC, Cobbold RC, Dohnt MF, Burns M-A o.a. High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires: intra-serovar divergence, inter-serovar convergence, and evidence of attenuation in Leptospira reference collections. Pathogens and Global Health. 2010 jul.;104(5):427-37. https://doi.org/10.1179/136485910X12786389891047

Author

Tulsiani, Suhella ; Craig, S B ; Graham, G C ; Cobbold, R C ; Dohnt, M F ; Burns, M-A ; Jansen, C C ; Leung, L K-P ; Field, H E ; Smythe, L D. / High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires : intra-serovar divergence, inter-serovar convergence, and evidence of attenuation in Leptospira reference collections. I: Pathogens and Global Health. 2010 ; Bind 104, Nr. 5. s. 427-37.

Bibtex

@article{395019805f8044baa5d4b212aba16f34,
title = "High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires: intra-serovar divergence, inter-serovar convergence, and evidence of attenuation in Leptospira reference collections",
abstract = "High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) is a novel technology that has emerged as a possible method to characterise leptospires to serovar level. RAPD-HRM has recently been used to measure intra-serovar convergence between strains of the same serovar as well as inter-serovar divergence between strains of different serovars. The results indicate that intra-serovar heterogeneity and inter-serovar homogeneity may limit the application of RAPD-HRM in routine diagnostics. They also indicate that genetic attenuation of aged, high-passage-number isolates could undermine the use of RAPD-HRM or any other molecular technology. Such genetic attenuation may account for a general decrease seen in titres of rabbit hyperimmune antibodies over time. Before RAPD-HRM can be further advanced as a routine diagnostic tool, strains more representative of the wild-type serovars of a given region need to be identified. Further, RAPD-HRM analysis of reference strains indicates that the routine renewal of reference collections, with new isolates, may be needed to maintain the genetic integrity of the collections.",
keywords = "Animals, DNA Fingerprinting, DNA, Bacterial, Humans, Leptospira, Leptospirosis, Mice, Random Amplified Polymorphic DNA Technique, Rats, Transition Temperature",
author = "Suhella Tulsiani and Craig, {S B} and Graham, {G C} and Cobbold, {R C} and Dohnt, {M F} and M-A Burns and Jansen, {C C} and Leung, {L K-P} and Field, {H E} and Smythe, {L D}",
year = "2010",
month = jul,
doi = "10.1179/136485910X12786389891047",
language = "English",
volume = "104",
pages = "427--37",
journal = "Pathogens and Global Health",
issn = "2047-7724",
publisher = "Taylor & Francis",
number = "5",

}

RIS

TY - JOUR

T1 - High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) for the characterisation of pathogenic leptospires

T2 - intra-serovar divergence, inter-serovar convergence, and evidence of attenuation in Leptospira reference collections

AU - Tulsiani, Suhella

AU - Craig, S B

AU - Graham, G C

AU - Cobbold, R C

AU - Dohnt, M F

AU - Burns, M-A

AU - Jansen, C C

AU - Leung, L K-P

AU - Field, H E

AU - Smythe, L D

PY - 2010/7

Y1 - 2010/7

N2 - High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) is a novel technology that has emerged as a possible method to characterise leptospires to serovar level. RAPD-HRM has recently been used to measure intra-serovar convergence between strains of the same serovar as well as inter-serovar divergence between strains of different serovars. The results indicate that intra-serovar heterogeneity and inter-serovar homogeneity may limit the application of RAPD-HRM in routine diagnostics. They also indicate that genetic attenuation of aged, high-passage-number isolates could undermine the use of RAPD-HRM or any other molecular technology. Such genetic attenuation may account for a general decrease seen in titres of rabbit hyperimmune antibodies over time. Before RAPD-HRM can be further advanced as a routine diagnostic tool, strains more representative of the wild-type serovars of a given region need to be identified. Further, RAPD-HRM analysis of reference strains indicates that the routine renewal of reference collections, with new isolates, may be needed to maintain the genetic integrity of the collections.

AB - High-resolution melt-curve analysis of random amplified polymorphic DNA (RAPD-HRM) is a novel technology that has emerged as a possible method to characterise leptospires to serovar level. RAPD-HRM has recently been used to measure intra-serovar convergence between strains of the same serovar as well as inter-serovar divergence between strains of different serovars. The results indicate that intra-serovar heterogeneity and inter-serovar homogeneity may limit the application of RAPD-HRM in routine diagnostics. They also indicate that genetic attenuation of aged, high-passage-number isolates could undermine the use of RAPD-HRM or any other molecular technology. Such genetic attenuation may account for a general decrease seen in titres of rabbit hyperimmune antibodies over time. Before RAPD-HRM can be further advanced as a routine diagnostic tool, strains more representative of the wild-type serovars of a given region need to be identified. Further, RAPD-HRM analysis of reference strains indicates that the routine renewal of reference collections, with new isolates, may be needed to maintain the genetic integrity of the collections.

KW - Animals

KW - DNA Fingerprinting

KW - DNA, Bacterial

KW - Humans

KW - Leptospira

KW - Leptospirosis

KW - Mice

KW - Random Amplified Polymorphic DNA Technique

KW - Rats

KW - Transition Temperature

U2 - 10.1179/136485910X12786389891047

DO - 10.1179/136485910X12786389891047

M3 - Journal article

C2 - 20819311

VL - 104

SP - 427

EP - 437

JO - Pathogens and Global Health

JF - Pathogens and Global Health

SN - 2047-7724

IS - 5

ER -

ID: 130477709