High throughput in vitro characterization of pectins for pig(let) nutrition
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High throughput in vitro characterization of pectins for pig(let) nutrition. / Wiese, Maria; Hui, Yan; Holck, Jesper; Sejberg, Jimmy J. P.; Daures, Celia; Maas, Evy; Kot, Witold; Borne, Johanna M.; Khakimov, Bekzod; Thymann, Thomas; Nielsen, Dennis Sandris.
I: BMC Animal Microbiome, Bind 3, 69, 2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - High throughput in vitro characterization of pectins for pig(let) nutrition
AU - Wiese, Maria
AU - Hui, Yan
AU - Holck, Jesper
AU - Sejberg, Jimmy J. P.
AU - Daures, Celia
AU - Maas, Evy
AU - Kot, Witold
AU - Borne, Johanna M.
AU - Khakimov, Bekzod
AU - Thymann, Thomas
AU - Nielsen, Dennis Sandris
PY - 2021
Y1 - 2021
N2 - BackgroundFiber-rich feed components possess prebiotic potential to enhance pig health and are considered a potential solution to the high prevalence of post-weaning diarrhea in pig production under the phased suspension of antibiotics and zinc oxide use.MethodsWe screened the gut microbiota modulatory properties of pectin substrates prepared from sugar beet within the freshly weaned piglet gut microbiome using an in vitro colon model, the CoMiniGut. We focused on testing a variety (13) of sugar beet-derived pectin substrates with defined structures, as well as known prebiotics such as inulin, fructooligosaccharide (FOS) and galactooligosaccharide (GOS), to gain insights on the structure–function related properties of specific substrates on the weaner gut microbial composition as well as shortchain fatty acid production (SCFA).ResultsSugar beet-derived pectin and rhamnogalacturonan-I selectively increased the relative abundance of Bacteroidetes, specifically Prevotella copri, Bacteroides ovatus, Bacteroides acidificiens, and an unclassified Bacteroides member. The degree of esterification impacted the relative abundance of these species and the SCFA production during the in vitro fermentations. Modified arabinans derived from sugar beet promoted the growth of Blautia, P. copri, Lachnospiraceae members and Limosilactobacillus mucosae and amongst all oligosaccharides tested yielded the highest amount of total SCFA produced after 24 h of fermentation. Sugar beet-derived substrates yielded higher total SCFA concentrations (especially acetic and propionic acid) relative to the known prebiotics inulin, FOS and GOS.ConclusionOur results indicate that the molecular structures of pectin, that can be prepared form just one plant source (sugar beet) can selectively stimulate different GM members, highlighting the potential of utilizing pectin substrates as targeted GM modulatory ingredients.
AB - BackgroundFiber-rich feed components possess prebiotic potential to enhance pig health and are considered a potential solution to the high prevalence of post-weaning diarrhea in pig production under the phased suspension of antibiotics and zinc oxide use.MethodsWe screened the gut microbiota modulatory properties of pectin substrates prepared from sugar beet within the freshly weaned piglet gut microbiome using an in vitro colon model, the CoMiniGut. We focused on testing a variety (13) of sugar beet-derived pectin substrates with defined structures, as well as known prebiotics such as inulin, fructooligosaccharide (FOS) and galactooligosaccharide (GOS), to gain insights on the structure–function related properties of specific substrates on the weaner gut microbial composition as well as shortchain fatty acid production (SCFA).ResultsSugar beet-derived pectin and rhamnogalacturonan-I selectively increased the relative abundance of Bacteroidetes, specifically Prevotella copri, Bacteroides ovatus, Bacteroides acidificiens, and an unclassified Bacteroides member. The degree of esterification impacted the relative abundance of these species and the SCFA production during the in vitro fermentations. Modified arabinans derived from sugar beet promoted the growth of Blautia, P. copri, Lachnospiraceae members and Limosilactobacillus mucosae and amongst all oligosaccharides tested yielded the highest amount of total SCFA produced after 24 h of fermentation. Sugar beet-derived substrates yielded higher total SCFA concentrations (especially acetic and propionic acid) relative to the known prebiotics inulin, FOS and GOS.ConclusionOur results indicate that the molecular structures of pectin, that can be prepared form just one plant source (sugar beet) can selectively stimulate different GM members, highlighting the potential of utilizing pectin substrates as targeted GM modulatory ingredients.
KW - Pectin
KW - Pectin structure
KW - Piglet
KW - Gut microbiome (GM)
KW - Weaning
KW - Short-chain fatty acids (SCFA)
KW - In vitro colon simulation
KW - Prebiotic
U2 - 10.1186/s42523-021-00129-w
DO - 10.1186/s42523-021-00129-w
M3 - Journal article
C2 - 34627409
VL - 3
JO - BMC Animal Microbiome
JF - BMC Animal Microbiome
SN - 2524-4671
M1 - 69
ER -
ID: 282191794