TY - JOUR

T1 - GTR-Mediated Radial Import Directs Accumulation of Defensive Glucosinolates to Sulfur-Rich Cells in the Phloem Cap of Arabidopsis Inflorescence Stem

AU - Xu, Deyang

AU - Hunziker, Pascal

AU - Koroleva, Olga

AU - Blennow, Andreas

AU - Crocoll, Christoph

AU - Schulz, Alexander

AU - Nour-Eldin, Hussam Hassan

AU - Halkier, Barbara Ann

PY - 2019

Y1 - 2019

N2 - In the phloem cap region of Arabidopsis, sulfur-rich cells (S-cells) accumulate >100 mM glucosinolates (GLS), but are biosynthetically inactive. The source and route of S-cell-bound GLS remain elusive. Here we used single-cell sampling and scanning electron microscopy with Energy-dispersive X-ray analysis to show that the GLS importers NPF2.10/GTR1 and NPF2.11/GTR2 are critical for GLS accumulation in S-cells, although not localized to the S-cells. Analysis of S-cell GLS in homo- and heterografts of gtr1 gtr2, and biosynthetic null mutant on wild type indicate that S-cells accumulate GLS via symplasmic connections either directly from neighboring biosynthetic cells or indirectly to non-neighboring cells expressing GTRs. Distinct sources and transport routes exist for different types of GLS, and vary dependent on the position of S-cells in the inflorescence stem. Based on our data, we propose a model for GLS transport routes either directly from biosynthetic cells or via GTR-mediated import from apoplastic space radially into a symplasmic domain wherein the S-cells are the ultimate sink. Similarly, we observed accumulation of the cyanogenic glucoside defense compounds in high-turgor cells in phloem cap of Lotus japonicus, suggesting that storage of defense compounds in high-turgor cells may be a general mechanism for chemical protection of the phloem cap.

AB - In the phloem cap region of Arabidopsis, sulfur-rich cells (S-cells) accumulate >100 mM glucosinolates (GLS), but are biosynthetically inactive. The source and route of S-cell-bound GLS remain elusive. Here we used single-cell sampling and scanning electron microscopy with Energy-dispersive X-ray analysis to show that the GLS importers NPF2.10/GTR1 and NPF2.11/GTR2 are critical for GLS accumulation in S-cells, although not localized to the S-cells. Analysis of S-cell GLS in homo- and heterografts of gtr1 gtr2, and biosynthetic null mutant on wild type indicate that S-cells accumulate GLS via symplasmic connections either directly from neighboring biosynthetic cells or indirectly to non-neighboring cells expressing GTRs. Distinct sources and transport routes exist for different types of GLS, and vary dependent on the position of S-cells in the inflorescence stem. Based on our data, we propose a model for GLS transport routes either directly from biosynthetic cells or via GTR-mediated import from apoplastic space radially into a symplasmic domain wherein the S-cells are the ultimate sink. Similarly, we observed accumulation of the cyanogenic glucoside defense compounds in high-turgor cells in phloem cap of Lotus japonicus, suggesting that storage of defense compounds in high-turgor cells may be a general mechanism for chemical protection of the phloem cap.

U2 - 10.1016/j.molp.2019.06.008

DO - 10.1016/j.molp.2019.06.008

M3 - Journal article

C2 - 31260813

VL - 12

SP - 1474

EP - 1484

JO - Molecular Plant

JF - Molecular Plant

SN - 1674-2052

IS - 11

ER -