Growth hormone is a growth factor for the differentiated pancreatic beta-cell

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Standard

Growth hormone is a growth factor for the differentiated pancreatic beta-cell. / Linde, S; Welinder, B S; Billestrup, N; Madsen, O D; Nielsen, Jens Høiriis.

I: Molecular endocrinology (Baltimore, Md.), Bind 3, Nr. 1, 01.1989, s. 165-73.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Linde, S, Welinder, BS, Billestrup, N, Madsen, OD & Nielsen, JH 1989, 'Growth hormone is a growth factor for the differentiated pancreatic beta-cell', Molecular endocrinology (Baltimore, Md.), bind 3, nr. 1, s. 165-73.

APA

Linde, S., Welinder, B. S., Billestrup, N., Madsen, O. D., & Nielsen, J. H. (1989). Growth hormone is a growth factor for the differentiated pancreatic beta-cell. Molecular endocrinology (Baltimore, Md.), 3(1), 165-73.

Vancouver

Linde S, Welinder BS, Billestrup N, Madsen OD, Nielsen JH. Growth hormone is a growth factor for the differentiated pancreatic beta-cell. Molecular endocrinology (Baltimore, Md.). 1989 jan;3(1):165-73.

Author

Linde, S ; Welinder, B S ; Billestrup, N ; Madsen, O D ; Nielsen, Jens Høiriis. / Growth hormone is a growth factor for the differentiated pancreatic beta-cell. I: Molecular endocrinology (Baltimore, Md.). 1989 ; Bind 3, Nr. 1. s. 165-73.

Bibtex

@article{31cbe58db3db40319fb5f35081a9c5f1,
title = "Growth hormone is a growth factor for the differentiated pancreatic beta-cell",
abstract = "The regulation of the growth of the pancreatic beta-cell is poorly understood. There are previous indications of a role of GH in the growth and insulin production of the pancreatic islets. In the present study we present evidence for a direct long-term effect of GH on proliferation and insulin biosynthesis of pancreatic beta-cells in monolayer culture. In culture medium RPMI 1640 supplemented with 2% normal human serum islets or dissociated islet cells from newborn rats maintained their insulin-producing capacity. When supplemented with 1-1000 ng/ml pituitary or recombinant human GH the islet cells attached, spread out, and proliferated into monolayers mainly consisting of insulin-containing cells. The number of beta-cells in S-phase was increased from 0.9-6.5% as determined by immunochemical staining of bromodeoxyuridine incorporated into insulin-positive cells. The increase in cell number was accompanied with a continuous increase in insulin release to the culture medium reaching a 10- 20-fold increase after 2-3 months with a half-maximal effect at about 10 ng/ml human GH. The biosynthesis of (pro)insulin was markedly increased with a normal rate of conversion of proinsulin to insulin. It is concluded that GH is a potent growth factor for the differentiated pancreatic beta-cell.",
keywords = "Animals, Animals, Newborn, Bromodeoxyuridine, Cell Division, Cells, Cultured, Chromatography, High Pressure Liquid, Growth Hormone, Immunohistochemistry, Insulin, Islets of Langerhans, Proinsulin, Rats, Rats, Inbred Strains, Recombinant Proteins",
author = "S Linde and Welinder, {B S} and N Billestrup and Madsen, {O D} and Nielsen, {Jens H{\o}iriis}",
year = "1989",
month = jan,
language = "English",
volume = "3",
pages = "165--73",
journal = "Molecular Endocrinology",
issn = "0888-8809",
publisher = "Oxford University Press",
number = "1",

}

RIS

TY - JOUR

T1 - Growth hormone is a growth factor for the differentiated pancreatic beta-cell

AU - Linde, S

AU - Welinder, B S

AU - Billestrup, N

AU - Madsen, O D

AU - Nielsen, Jens Høiriis

PY - 1989/1

Y1 - 1989/1

N2 - The regulation of the growth of the pancreatic beta-cell is poorly understood. There are previous indications of a role of GH in the growth and insulin production of the pancreatic islets. In the present study we present evidence for a direct long-term effect of GH on proliferation and insulin biosynthesis of pancreatic beta-cells in monolayer culture. In culture medium RPMI 1640 supplemented with 2% normal human serum islets or dissociated islet cells from newborn rats maintained their insulin-producing capacity. When supplemented with 1-1000 ng/ml pituitary or recombinant human GH the islet cells attached, spread out, and proliferated into monolayers mainly consisting of insulin-containing cells. The number of beta-cells in S-phase was increased from 0.9-6.5% as determined by immunochemical staining of bromodeoxyuridine incorporated into insulin-positive cells. The increase in cell number was accompanied with a continuous increase in insulin release to the culture medium reaching a 10- 20-fold increase after 2-3 months with a half-maximal effect at about 10 ng/ml human GH. The biosynthesis of (pro)insulin was markedly increased with a normal rate of conversion of proinsulin to insulin. It is concluded that GH is a potent growth factor for the differentiated pancreatic beta-cell.

AB - The regulation of the growth of the pancreatic beta-cell is poorly understood. There are previous indications of a role of GH in the growth and insulin production of the pancreatic islets. In the present study we present evidence for a direct long-term effect of GH on proliferation and insulin biosynthesis of pancreatic beta-cells in monolayer culture. In culture medium RPMI 1640 supplemented with 2% normal human serum islets or dissociated islet cells from newborn rats maintained their insulin-producing capacity. When supplemented with 1-1000 ng/ml pituitary or recombinant human GH the islet cells attached, spread out, and proliferated into monolayers mainly consisting of insulin-containing cells. The number of beta-cells in S-phase was increased from 0.9-6.5% as determined by immunochemical staining of bromodeoxyuridine incorporated into insulin-positive cells. The increase in cell number was accompanied with a continuous increase in insulin release to the culture medium reaching a 10- 20-fold increase after 2-3 months with a half-maximal effect at about 10 ng/ml human GH. The biosynthesis of (pro)insulin was markedly increased with a normal rate of conversion of proinsulin to insulin. It is concluded that GH is a potent growth factor for the differentiated pancreatic beta-cell.

KW - Animals

KW - Animals, Newborn

KW - Bromodeoxyuridine

KW - Cell Division

KW - Cells, Cultured

KW - Chromatography, High Pressure Liquid

KW - Growth Hormone

KW - Immunohistochemistry

KW - Insulin

KW - Islets of Langerhans

KW - Proinsulin

KW - Rats

KW - Rats, Inbred Strains

KW - Recombinant Proteins

M3 - Journal article

C2 - 2644530

VL - 3

SP - 165

EP - 173

JO - Molecular Endocrinology

JF - Molecular Endocrinology

SN - 0888-8809

IS - 1

ER -

ID: 47974458