Growth hormone is a growth factor for the differentiated pancreatic beta-cell
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Growth hormone is a growth factor for the differentiated pancreatic beta-cell. / Linde, S; Welinder, B S; Billestrup, N; Madsen, O D; Nielsen, Jens Høiriis.
I: Molecular endocrinology (Baltimore, Md.), Bind 3, Nr. 1, 01.1989, s. 165-73.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Growth hormone is a growth factor for the differentiated pancreatic beta-cell
AU - Linde, S
AU - Welinder, B S
AU - Billestrup, N
AU - Madsen, O D
AU - Nielsen, Jens Høiriis
PY - 1989/1
Y1 - 1989/1
N2 - The regulation of the growth of the pancreatic beta-cell is poorly understood. There are previous indications of a role of GH in the growth and insulin production of the pancreatic islets. In the present study we present evidence for a direct long-term effect of GH on proliferation and insulin biosynthesis of pancreatic beta-cells in monolayer culture. In culture medium RPMI 1640 supplemented with 2% normal human serum islets or dissociated islet cells from newborn rats maintained their insulin-producing capacity. When supplemented with 1-1000 ng/ml pituitary or recombinant human GH the islet cells attached, spread out, and proliferated into monolayers mainly consisting of insulin-containing cells. The number of beta-cells in S-phase was increased from 0.9-6.5% as determined by immunochemical staining of bromodeoxyuridine incorporated into insulin-positive cells. The increase in cell number was accompanied with a continuous increase in insulin release to the culture medium reaching a 10- 20-fold increase after 2-3 months with a half-maximal effect at about 10 ng/ml human GH. The biosynthesis of (pro)insulin was markedly increased with a normal rate of conversion of proinsulin to insulin. It is concluded that GH is a potent growth factor for the differentiated pancreatic beta-cell.
AB - The regulation of the growth of the pancreatic beta-cell is poorly understood. There are previous indications of a role of GH in the growth and insulin production of the pancreatic islets. In the present study we present evidence for a direct long-term effect of GH on proliferation and insulin biosynthesis of pancreatic beta-cells in monolayer culture. In culture medium RPMI 1640 supplemented with 2% normal human serum islets or dissociated islet cells from newborn rats maintained their insulin-producing capacity. When supplemented with 1-1000 ng/ml pituitary or recombinant human GH the islet cells attached, spread out, and proliferated into monolayers mainly consisting of insulin-containing cells. The number of beta-cells in S-phase was increased from 0.9-6.5% as determined by immunochemical staining of bromodeoxyuridine incorporated into insulin-positive cells. The increase in cell number was accompanied with a continuous increase in insulin release to the culture medium reaching a 10- 20-fold increase after 2-3 months with a half-maximal effect at about 10 ng/ml human GH. The biosynthesis of (pro)insulin was markedly increased with a normal rate of conversion of proinsulin to insulin. It is concluded that GH is a potent growth factor for the differentiated pancreatic beta-cell.
KW - Animals
KW - Animals, Newborn
KW - Bromodeoxyuridine
KW - Cell Division
KW - Cells, Cultured
KW - Chromatography, High Pressure Liquid
KW - Growth Hormone
KW - Immunohistochemistry
KW - Insulin
KW - Islets of Langerhans
KW - Proinsulin
KW - Rats
KW - Rats, Inbred Strains
KW - Recombinant Proteins
M3 - Journal article
C2 - 2644530
VL - 3
SP - 165
EP - 173
JO - Molecular Endocrinology
JF - Molecular Endocrinology
SN - 0888-8809
IS - 1
ER -
ID: 47974458