Glucagon-like peptide I receptors in the subfornical organ and the area postrema are accessible to circulating glucagon-like peptide I

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Standard

Glucagon-like peptide I receptors in the subfornical organ and the area postrema are accessible to circulating glucagon-like peptide I. / Orskov, C; Poulsen, Steen Seier; Møller, M; Holst, J J.

I: Diabetes, Bind 45, Nr. 6, 06.1996, s. 832-5.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Orskov, C, Poulsen, SS, Møller, M & Holst, JJ 1996, 'Glucagon-like peptide I receptors in the subfornical organ and the area postrema are accessible to circulating glucagon-like peptide I', Diabetes, bind 45, nr. 6, s. 832-5.

APA

Orskov, C., Poulsen, S. S., Møller, M., & Holst, J. J. (1996). Glucagon-like peptide I receptors in the subfornical organ and the area postrema are accessible to circulating glucagon-like peptide I. Diabetes, 45(6), 832-5.

Vancouver

Orskov C, Poulsen SS, Møller M, Holst JJ. Glucagon-like peptide I receptors in the subfornical organ and the area postrema are accessible to circulating glucagon-like peptide I. Diabetes. 1996 jun.;45(6):832-5.

Author

Orskov, C ; Poulsen, Steen Seier ; Møller, M ; Holst, J J. / Glucagon-like peptide I receptors in the subfornical organ and the area postrema are accessible to circulating glucagon-like peptide I. I: Diabetes. 1996 ; Bind 45, Nr. 6. s. 832-5.

Bibtex

@article{16c8d370a2ec40fa9dbe6d3b15b2c9e1,

title = "Glucagon-like peptide I receptors in the subfornical organ and the area postrema are accessible to circulating glucagon-like peptide I",

abstract = "The intestinal incretin hormone glucagon-like peptide I (GLP-I) inhibits gastric motility and secretion in normal, but not in vagotomized subjects, pointing to a centrally mediated effect. Therefore, our aim was to study the availability of rat brain GLP-I receptors to peripherally injected 125I-labeled GLP-I. The specificity of the binding was tested by co-injection of excess amounts of unlabeled GLP-I. Using light microscopical autoradiography of rat brain sections, we found specific 125I-GLP-I binding exclusively in the subfornical organ and the area postrema. This binding was abolished when an excess amount of unlabeled GLP-I was co-injected with the labeled GLP-I. We conclude that cells in the subfornical organ and the area postrema could be responsive to blood-borne GLP-I. The observed binding of peripherally administered GLP-I to the subfornical organ and the area postrema, which both have close neuroanatomical connections with hypothalamic areas involved in water and appetite homeostasis, is consistent with the potential roles of circulating GLP-I in the central regulation of appetite and autonomic functions.",

keywords = "Animals, Autoradiography, Cerebral Ventricles, Female, Glucagon-Like Peptides, Hypothalamus, Iodine Radioisotopes, Peptides, Protein Precursors, Rats, Rats, Wistar, Receptors, Glucagon, Subfornical Organ",

author = "C Orskov and Poulsen, {Steen Seier} and M M{\o}ller and Holst, {J J}",

year = "1996",

month = jun,

language = "English",

volume = "45",

pages = "832--5",

journal = "Diabetes",

issn = "0012-1797",

publisher = "American Diabetes Association",

number = "6",

}

RIS

TY - JOUR

T1 - Glucagon-like peptide I receptors in the subfornical organ and the area postrema are accessible to circulating glucagon-like peptide I

AU - Orskov, C

AU - Poulsen, Steen Seier

AU - Møller, M

AU - Holst, J J

PY - 1996/6

Y1 - 1996/6

N2 - The intestinal incretin hormone glucagon-like peptide I (GLP-I) inhibits gastric motility and secretion in normal, but not in vagotomized subjects, pointing to a centrally mediated effect. Therefore, our aim was to study the availability of rat brain GLP-I receptors to peripherally injected 125I-labeled GLP-I. The specificity of the binding was tested by co-injection of excess amounts of unlabeled GLP-I. Using light microscopical autoradiography of rat brain sections, we found specific 125I-GLP-I binding exclusively in the subfornical organ and the area postrema. This binding was abolished when an excess amount of unlabeled GLP-I was co-injected with the labeled GLP-I. We conclude that cells in the subfornical organ and the area postrema could be responsive to blood-borne GLP-I. The observed binding of peripherally administered GLP-I to the subfornical organ and the area postrema, which both have close neuroanatomical connections with hypothalamic areas involved in water and appetite homeostasis, is consistent with the potential roles of circulating GLP-I in the central regulation of appetite and autonomic functions.

AB - The intestinal incretin hormone glucagon-like peptide I (GLP-I) inhibits gastric motility and secretion in normal, but not in vagotomized subjects, pointing to a centrally mediated effect. Therefore, our aim was to study the availability of rat brain GLP-I receptors to peripherally injected 125I-labeled GLP-I. The specificity of the binding was tested by co-injection of excess amounts of unlabeled GLP-I. Using light microscopical autoradiography of rat brain sections, we found specific 125I-GLP-I binding exclusively in the subfornical organ and the area postrema. This binding was abolished when an excess amount of unlabeled GLP-I was co-injected with the labeled GLP-I. We conclude that cells in the subfornical organ and the area postrema could be responsive to blood-borne GLP-I. The observed binding of peripherally administered GLP-I to the subfornical organ and the area postrema, which both have close neuroanatomical connections with hypothalamic areas involved in water and appetite homeostasis, is consistent with the potential roles of circulating GLP-I in the central regulation of appetite and autonomic functions.

KW - Animals

KW - Autoradiography

KW - Cerebral Ventricles

KW - Female

KW - Glucagon-Like Peptides

KW - Hypothalamus

KW - Iodine Radioisotopes

KW - Peptides

KW - Protein Precursors

KW - Rats

KW - Rats, Wistar

KW - Receptors, Glucagon

KW - Subfornical Organ

M3 - Journal article

C2 - 8635662

VL - 45

SP - 832

EP - 835

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 6

ER -

ID: 47486835