Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus
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The human MDR1 gene encodes the efflux transporter P-glycoprotein, which plays an important part of the blood-brain barrier function of brain microvascular endothelial cells (BMECs). Here, we report the generation of an iPSC line, where a construct of the human MDR1 gene was inserted into the safe-site locus AAVS1. This iPSC line (BIONi010-C-48) shows functional expression of P-gp and can further be differentiated and cultured into electrically tight BMEC-like monolayers exhibiting polarized expression of P-gp in the apical membrane.
Originalsprog | Engelsk |
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Artikelnummer | 103348 |
Tidsskrift | Stem Cell Research |
Vol/bind | 76 |
Antal sider | 5 |
ISSN | 1873-5061 |
DOI | |
Status | Udgivet - 2024 |
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Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
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