Garlic-Derived Metabolites Exert Antioxidant Activity, Modulate Gut Microbiota Composition and Limit Citrobacter rodentium Infection in Mice

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Garlic-Derived Metabolites Exert Antioxidant Activity, Modulate Gut Microbiota Composition and Limit Citrobacter rodentium Infection in Mice. / Zhu, Ling; Andersen-Civil, Audrey I.S.; Castro-Meija, Josue L.; Nielsen, Dennis S.; Blanchard, Alexandra; Olsen, John E.; Thamsborg, Stig M.; Williams, Andrew R.

I: Antioxidants, Bind 11, Nr. 10, 2033, 2022.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Zhu, L, Andersen-Civil, AIS, Castro-Meija, JL, Nielsen, DS, Blanchard, A, Olsen, JE, Thamsborg, SM & Williams, AR 2022, 'Garlic-Derived Metabolites Exert Antioxidant Activity, Modulate Gut Microbiota Composition and Limit Citrobacter rodentium Infection in Mice', Antioxidants, bind 11, nr. 10, 2033. https://doi.org/10.3390/antiox11102033

APA

Zhu, L., Andersen-Civil, A. I. S., Castro-Meija, J. L., Nielsen, D. S., Blanchard, A., Olsen, J. E., Thamsborg, S. M., & Williams, A. R. (2022). Garlic-Derived Metabolites Exert Antioxidant Activity, Modulate Gut Microbiota Composition and Limit Citrobacter rodentium Infection in Mice. Antioxidants, 11(10), [2033]. https://doi.org/10.3390/antiox11102033

Vancouver

Zhu L, Andersen-Civil AIS, Castro-Meija JL, Nielsen DS, Blanchard A, Olsen JE o.a. Garlic-Derived Metabolites Exert Antioxidant Activity, Modulate Gut Microbiota Composition and Limit Citrobacter rodentium Infection in Mice. Antioxidants. 2022;11(10). 2033. https://doi.org/10.3390/antiox11102033

Author

Zhu, Ling ; Andersen-Civil, Audrey I.S. ; Castro-Meija, Josue L. ; Nielsen, Dennis S. ; Blanchard, Alexandra ; Olsen, John E. ; Thamsborg, Stig M. ; Williams, Andrew R. / Garlic-Derived Metabolites Exert Antioxidant Activity, Modulate Gut Microbiota Composition and Limit Citrobacter rodentium Infection in Mice. I: Antioxidants. 2022 ; Bind 11, Nr. 10.

Bibtex

@article{8898208166bf495583d6587b5be8cdac,
title = "Garlic-Derived Metabolites Exert Antioxidant Activity, Modulate Gut Microbiota Composition and Limit Citrobacter rodentium Infection in Mice",
abstract = "The garlic-derived compounds propyl propane thiosulfinate (PTS) and propyl propane thiosulfonate (PTSO) are metabolites with putative health benefits against intestinal inflammation that may be related to their antioxidant activity. However, the underlying mechanisms remain unclear, and whether PTS-PTSO can promote gut health by altering the microbiota and exert protection against enteric pathogens needs further investigation. Here, we explored the antioxidant activity of PTS-PTSO in murine macrophages in vitro, and in an in vivo model of bacterial infection with the bacterial pathogen Citrobacter rodentium. PTS-PTSO attenuated reactive oxygen species in lipopolysaccharide-stimulated macrophages in a nuclear factor erythroid factor 2-related factor 2 (Nrf2)-dependent manner, decreased nitric oxide levels both in macrophages in vitro and in the sera of mice fed PTS-PTSO, and had putatively beneficial effects on the commensal gut microbiota. Importantly, PTS-PTSO decreased faecal C. rodentium counts, concomitant with upregulation of Nrf2-related genes in colon tissue. Thus, PTS-PTSO mediates Nrf2-mediated antioxidant activity and modulates gut microbiota, which may protect the host against C. rodentium colonization. Our results provide further insight into how PTS-PTSO and related bioactive dietary compounds may reduce enteric infections.",
keywords = "antioxidant activity, Citrobacter rodentium, nuclear factor erythroid factor 2-related factor 2, propyl propane thiosulfinate, propyl propane thiosulfonate",
author = "Ling Zhu and Andersen-Civil, {Audrey I.S.} and Castro-Meija, {Josue L.} and Nielsen, {Dennis S.} and Alexandra Blanchard and Olsen, {John E.} and Thamsborg, {Stig M.} and Williams, {Andrew R.}",
note = "Publisher Copyright: {\textcopyright} 2022 by the authors.",
year = "2022",
doi = "10.3390/antiox11102033",
language = "English",
volume = "11",
journal = "Antioxidants",
issn = "2076-3921",
publisher = "M D P I AG",
number = "10",

}

RIS

TY - JOUR

T1 - Garlic-Derived Metabolites Exert Antioxidant Activity, Modulate Gut Microbiota Composition and Limit Citrobacter rodentium Infection in Mice

AU - Zhu, Ling

AU - Andersen-Civil, Audrey I.S.

AU - Castro-Meija, Josue L.

AU - Nielsen, Dennis S.

AU - Blanchard, Alexandra

AU - Olsen, John E.

AU - Thamsborg, Stig M.

AU - Williams, Andrew R.

N1 - Publisher Copyright: © 2022 by the authors.

PY - 2022

Y1 - 2022

N2 - The garlic-derived compounds propyl propane thiosulfinate (PTS) and propyl propane thiosulfonate (PTSO) are metabolites with putative health benefits against intestinal inflammation that may be related to their antioxidant activity. However, the underlying mechanisms remain unclear, and whether PTS-PTSO can promote gut health by altering the microbiota and exert protection against enteric pathogens needs further investigation. Here, we explored the antioxidant activity of PTS-PTSO in murine macrophages in vitro, and in an in vivo model of bacterial infection with the bacterial pathogen Citrobacter rodentium. PTS-PTSO attenuated reactive oxygen species in lipopolysaccharide-stimulated macrophages in a nuclear factor erythroid factor 2-related factor 2 (Nrf2)-dependent manner, decreased nitric oxide levels both in macrophages in vitro and in the sera of mice fed PTS-PTSO, and had putatively beneficial effects on the commensal gut microbiota. Importantly, PTS-PTSO decreased faecal C. rodentium counts, concomitant with upregulation of Nrf2-related genes in colon tissue. Thus, PTS-PTSO mediates Nrf2-mediated antioxidant activity and modulates gut microbiota, which may protect the host against C. rodentium colonization. Our results provide further insight into how PTS-PTSO and related bioactive dietary compounds may reduce enteric infections.

AB - The garlic-derived compounds propyl propane thiosulfinate (PTS) and propyl propane thiosulfonate (PTSO) are metabolites with putative health benefits against intestinal inflammation that may be related to their antioxidant activity. However, the underlying mechanisms remain unclear, and whether PTS-PTSO can promote gut health by altering the microbiota and exert protection against enteric pathogens needs further investigation. Here, we explored the antioxidant activity of PTS-PTSO in murine macrophages in vitro, and in an in vivo model of bacterial infection with the bacterial pathogen Citrobacter rodentium. PTS-PTSO attenuated reactive oxygen species in lipopolysaccharide-stimulated macrophages in a nuclear factor erythroid factor 2-related factor 2 (Nrf2)-dependent manner, decreased nitric oxide levels both in macrophages in vitro and in the sera of mice fed PTS-PTSO, and had putatively beneficial effects on the commensal gut microbiota. Importantly, PTS-PTSO decreased faecal C. rodentium counts, concomitant with upregulation of Nrf2-related genes in colon tissue. Thus, PTS-PTSO mediates Nrf2-mediated antioxidant activity and modulates gut microbiota, which may protect the host against C. rodentium colonization. Our results provide further insight into how PTS-PTSO and related bioactive dietary compounds may reduce enteric infections.

KW - antioxidant activity

KW - Citrobacter rodentium

KW - nuclear factor erythroid factor 2-related factor 2

KW - propyl propane thiosulfinate

KW - propyl propane thiosulfonate

U2 - 10.3390/antiox11102033

DO - 10.3390/antiox11102033

M3 - Journal article

C2 - 36290756

AN - SCOPUS:85140465921

VL - 11

JO - Antioxidants

JF - Antioxidants

SN - 2076-3921

IS - 10

M1 - 2033

ER -

ID: 324309918