TY - JOUR

T1 - FIND-IT

T2 - Accelerated trait development for a green evolution

AU - Knudsen, Søren

AU - Wendt, Toni

AU - Dockter, Christoph

AU - Thomsen, Hanne Cecilie

AU - Rasmussen, Magnus

AU - Jørgensen, Morten Egevang

AU - Lu, Qiongxian

AU - Voss, Cynthia

AU - Murozuka, Emiko

AU - Østerberg, Jeppe Thulin

AU - Harholt, Jesper

AU - Braumann, Ilka

AU - Cuesta-Seijo, Jose A.

AU - Kale, Sandip M.

AU - Bodevin, Sabrina

AU - Tang Petersen, Lise

AU - Carciofi, Massimiliano

AU - Pedas, Pai Rosager

AU - Opstrup Husum, Jeppe

AU - Nielsen, Martin Toft Simmelsgaard

AU - Nielsen, Kasper

AU - Jensen, Mikkel K.

AU - Møller, Lillian Ambus

AU - Gojkovic, Zoran

AU - Striebeck, Alexander

AU - Lengeler, Klaus

AU - Fennessy, Ross T.

AU - Katz, Michael

AU - Garcia Sanchez, Rosa

AU - Solodovnikova, Natalia

AU - Förster, Jochen

AU - Olsen, Ole

AU - Møller, Birger Lindberg

AU - Fincher, Geoffrey B.

AU - Skadhauge, Birgitte

PY - 2022

Y1 - 2022

N2 - Improved agricultural and industrial production organisms are required to meet the future global food demands and minimize the effects of climate change. A new resource for crop and microbe improvement, designated FIND-IT (Fast Identification of Nucleotide variants by droplet DigITal PCR), provides ultrafast identification and isolation of predetermined, targeted genetic variants in a screening cycle of less than 10 days. Using large-scale sample pooling in combination with droplet digital PCR (ddPCR) greatly increases the size of low-mutation density and screenable variant libraries and the probability of identifying the variant of interest. The method is validated by screening variant libraries totaling 500,000 barley (Hordeum vulgare) individuals and isolating more than 125 targeted barley gene knockout lines and miRNA or promoter variants enabling functional gene analysis. FIND-IT variants are directly applicable to elite breeding pipelines and minimize time-consuming technical steps to accelerate the evolution of germplasm.

AB - Improved agricultural and industrial production organisms are required to meet the future global food demands and minimize the effects of climate change. A new resource for crop and microbe improvement, designated FIND-IT (Fast Identification of Nucleotide variants by droplet DigITal PCR), provides ultrafast identification and isolation of predetermined, targeted genetic variants in a screening cycle of less than 10 days. Using large-scale sample pooling in combination with droplet digital PCR (ddPCR) greatly increases the size of low-mutation density and screenable variant libraries and the probability of identifying the variant of interest. The method is validated by screening variant libraries totaling 500,000 barley (Hordeum vulgare) individuals and isolating more than 125 targeted barley gene knockout lines and miRNA or promoter variants enabling functional gene analysis. FIND-IT variants are directly applicable to elite breeding pipelines and minimize time-consuming technical steps to accelerate the evolution of germplasm.

U2 - 10.1126/sciadv.abq2266

DO - 10.1126/sciadv.abq2266

M3 - Journal article

C2 - 36001660

AN - SCOPUS:85136525271

VL - 8

JO - Science advances

JF - Science advances

SN - 2375-2548

IS - 34

M1 - eabq2266

ER -