Expression of the sarcoplasmic reticulum Ca2+-ATPase in yeast
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
We describe here an easy system for the production of mg amounts of the rabbit Ca2+-ATPase SERCA 1a in the yeast S. cerevisiae. The protein is present in several membranes, including the plasma membrane of the yeast, in a native conformation. It can be purified by immunoprecipitation and can be phosphorylated from ATP in a Ca2+-dependent manner. Using a temperature-sensitive secretion mutant strain, the fully active protein can also be obtained in secretory vesicles.
Originalsprog | Engelsk |
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Tidsskrift | FEBS Letters |
Vol/bind | 354 |
Udgave nummer | 1 |
Sider (fra-til) | 117-122 |
Antal sider | 6 |
ISSN | 0014-5793 |
DOI | |
Status | Udgivet - 31 okt. 1994 |
ID: 245002833