Expression of the growth hormone receptor gene in insulin producing cells
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Expression of the growth hormone receptor gene in insulin producing cells. / Møldrup, Annette; Billestrup, N; Nielsen, Jens Høiriis.
I: Biomedica Biochimica Acta, Bind 49, Nr. 12, 1990, s. 1151-5.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Expression of the growth hormone receptor gene in insulin producing cells
AU - Møldrup, Annette
AU - Billestrup, N
AU - Nielsen, Jens Høiriis
PY - 1990
Y1 - 1990
N2 - Growth hormone (GH) plays a dual role in glucose homeostasis. On the one hand, it exerts an insulin antagonistic effect on the peripheral tissue, on the other hand, it stimulates insulin biosynthesis and beta-cell proliferation. The expression of GH-receptors on the rat insulinoma cell line RIN-5AH-T2-clone B was studied. The binding characteristics with regard to specificity for the native 22 kDa hGH, and the 20 kDa variant were similar to that reported on rat adipocytes. Normal rat islet cells showed a similar affinity for hGH. The RIN cells express GH receptors similar to the cloned liver receptor. It is hypothesized that defects in the receptor expression on the beta-cells may contribute to the susceptibility to develop diabetes.
AB - Growth hormone (GH) plays a dual role in glucose homeostasis. On the one hand, it exerts an insulin antagonistic effect on the peripheral tissue, on the other hand, it stimulates insulin biosynthesis and beta-cell proliferation. The expression of GH-receptors on the rat insulinoma cell line RIN-5AH-T2-clone B was studied. The binding characteristics with regard to specificity for the native 22 kDa hGH, and the 20 kDa variant were similar to that reported on rat adipocytes. Normal rat islet cells showed a similar affinity for hGH. The RIN cells express GH receptors similar to the cloned liver receptor. It is hypothesized that defects in the receptor expression on the beta-cells may contribute to the susceptibility to develop diabetes.
KW - Animals
KW - Binding, Competitive
KW - Cell Line
KW - Chromatography, High Pressure Liquid
KW - Gene Expression Regulation
KW - Insulin
KW - Islets of Langerhans
KW - Rats
KW - Receptors, Somatotropin
KW - Tumor Cells, Cultured
M3 - Journal article
C2 - 2097987
VL - 49
SP - 1151
EP - 1155
JO - Biomedica Biochimica Acta
JF - Biomedica Biochimica Acta
SN - 0232-766X
IS - 12
ER -
ID: 47974193