Expression of endothelin type B receptors in uterine artery smooth muscle cells from pregnant Guinea pigs
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Expression of endothelin type B receptors in uterine artery smooth muscle cells from pregnant Guinea pigs. / Skovsted, Gry Freja; Tveden-Nyborg, Pernille; Lykkesfeldt, Jens.
I: Placenta, Bind 77, 2019, s. 8-15.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Expression of endothelin type B receptors in uterine artery smooth muscle cells from pregnant Guinea pigs
AU - Skovsted, Gry Freja
AU - Tveden-Nyborg, Pernille
AU - Lykkesfeldt, Jens
PY - 2019
Y1 - 2019
N2 - Introduction: It is well established that upregulation of endothelin type B (ET B ) receptors in vascular smooth muscle cells plays a role in pathophysiological artery remodeling as response to ischemia and atherosclerosis. This study aimed to investigate the ET B receptors function and localization under normal physiological remodeling. Specifically, in the guinea pig uterine arteries during pregnancy. Methods: Uterine artery contractility was assessed with sarafotoxin 6c and endothelin-1 in wire-myography in uterine arteries from non-pregnant and pregnant guinea pigs at gestational day 37 ± 5. Localization of ET B receptors, proliferation marker Ki-67, and SMC differentiation marker SM22α in uterine arteries were investigated with immunohistochemistry. Results: Uterine arteries from pregnant guinea pigs showed significantly increased ET B receptor-mediated vasoconstriction compared to uterine arteries from non-pregnant and to coronary arteries from pregnant guinea pigs (p < 0.001), suggesting that ET B -receptor upregulation in uterine artery SMCs is a normal physiological mechanism taking place during remodeling. Furthermore, uterine arteries from pregnant guinea pigs showed enhanced expression of ET B receptors, high density of Ki-67 positive SMCs and sparse SM22α staining in SMCs localized in the outer layer of the vessel wall. Discussion: Our results suggest that ET B receptors are expressed in dedifferentiated proliferating SMCs of uterine arteries in pregnant guinea pigs. This study provides novel insight into the function and expression of ET B receptors in uterine vascular remodeling during pregnancy.
AB - Introduction: It is well established that upregulation of endothelin type B (ET B ) receptors in vascular smooth muscle cells plays a role in pathophysiological artery remodeling as response to ischemia and atherosclerosis. This study aimed to investigate the ET B receptors function and localization under normal physiological remodeling. Specifically, in the guinea pig uterine arteries during pregnancy. Methods: Uterine artery contractility was assessed with sarafotoxin 6c and endothelin-1 in wire-myography in uterine arteries from non-pregnant and pregnant guinea pigs at gestational day 37 ± 5. Localization of ET B receptors, proliferation marker Ki-67, and SMC differentiation marker SM22α in uterine arteries were investigated with immunohistochemistry. Results: Uterine arteries from pregnant guinea pigs showed significantly increased ET B receptor-mediated vasoconstriction compared to uterine arteries from non-pregnant and to coronary arteries from pregnant guinea pigs (p < 0.001), suggesting that ET B -receptor upregulation in uterine artery SMCs is a normal physiological mechanism taking place during remodeling. Furthermore, uterine arteries from pregnant guinea pigs showed enhanced expression of ET B receptors, high density of Ki-67 positive SMCs and sparse SM22α staining in SMCs localized in the outer layer of the vessel wall. Discussion: Our results suggest that ET B receptors are expressed in dedifferentiated proliferating SMCs of uterine arteries in pregnant guinea pigs. This study provides novel insight into the function and expression of ET B receptors in uterine vascular remodeling during pregnancy.
U2 - 10.1016/j.placenta.2019.01.015
DO - 10.1016/j.placenta.2019.01.015
M3 - Journal article
C2 - 30827357
AN - SCOPUS:85060692534
VL - 77
SP - 8
EP - 15
JO - Placenta
JF - Placenta
SN - 0143-4004
ER -
ID: 216932159